单位：[1]Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing 100049, China[2]Department of Radiation Oncology, Hunan Provincial Tumor Hospital and Affiliated Tumor Hospital ofXiangya Medical School, Central South University, Changsha 410013, China[3]Key Laboratory of Particle and Radiation Imaging (Tsinghua University), Ministry of Education, Beijing 100084, China[4]State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences,University of Macau, Macao 999078, China[5]Department of Integrated Traditional and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China
In order to investigate whether cell organelle targeting peptide can transport magnetic nanoparticles (MNPs) into specific cell organelle, peptides bearing nuclear localization signal (NLS) or mitochondria targeting sequences were coagulated to MNPs. In vitro cytotoxicity study on the human liver cancer cells (HepG2) was tested by using MTT assay. Sub-cellular location of each peptide modified MNP (PEP-MNPs) was observed by transmission electron microscopy (TEM). The uptake of HepG2 cells growing in PEP-MNPs was measured by using ICP-OES. Magnetic induction heating efficacies of PEP-MNPs were analyzed by exposing the PEP-MNPs containing cells in an alternating magnetic field (AMF). It was demonstrated that PEP-MNPs were efficient agents for cancer nanothermotherapy with satisfactory biocompatibility. TEM showed that the fate of MNPs inside the cells depended on the peptide sequence attached to the particle surface. The uptake improvement was observed both in PEP-MNPs bearing NLS peptides and in PEP-MNPs bearing mitochondria targeting sequences. Virus original endocytosis sequence can enhance the uptake. MNP bearing mitochondria targeting sequence exerted a better magnetic induction hyperthermia performance comparing to that of NLS. Our investigation provides a strategy for fabrication cell organelle targeting magnetic nanoparticles. For instance, mitochondria targeting peptide conjugated MNPs for highly-efficiency magnetic nanothermotherapy and nuclear targeting peptides conjugated MNPs for gene magnetofection.
基金：
China National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81071885, 10775085]
第一作者单位：[1]Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing 100049, China
通讯作者：
通讯机构：[1]Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing 100049, China[3]Key Laboratory of Particle and Radiation Imaging (Tsinghua University), Ministry of Education, Beijing 100084, China
推荐引用方式(GB/T 7714)：
Wang Xiaowen,Zhou Jumei,Chen Benke,et al.Enhanced Intracellular Hyperthermia Efficiency by Magnetic Nanoparticles Modified with Nucleus and Mitochondria Targeting Peptides[J].JOURNAL of NANOSCIENCE and NANOTECHNOLOGY.2016,16(6):6560-6566.doi:10.1166/jnn.2016.10852.
APA：
Wang, Xiaowen,Zhou, Jumei,Chen, Benke,Tang, Zhenghai,Zhang, Jieying...&Tang, Jintian.(2016).Enhanced Intracellular Hyperthermia Efficiency by Magnetic Nanoparticles Modified with Nucleus and Mitochondria Targeting Peptides.JOURNAL of NANOSCIENCE and NANOTECHNOLOGY,16,(6)
MLA：
Wang, Xiaowen,et al."Enhanced Intracellular Hyperthermia Efficiency by Magnetic Nanoparticles Modified with Nucleus and Mitochondria Targeting Peptides".JOURNAL of NANOSCIENCE and NANOTECHNOLOGY 16..6(2016):6560-6566
