单位：[1]Department of Pediatrics, China-Japan Friendship Hospital, Beijing, China[2]Clinical Research Institute, China-Japan Friendship Hospital, Beijing, China[3]Department of Laboratory Medicine, China-Japan Friendship Hospital, Beijing, China
Traditional Chinese medicine QPYF has a good effect for treating antibiotic-associated diarrhea in clinical practice. The aim of this study is to test its efficacy to prevent Clostridium difficile associated diarrhea (CDAD) in a mouse model. C57BL/6 mice were infected with Clostridium difficile VPI 10463 after exposure to antimicrobial mixture. QPYF was administered from 7 days prior to Clostridium difficile infection to 20 days after infection, and its effect was compared with no treatment and receiving placebo. The mice were monitored for 20 days and the percent survival, disease activity index, weight loss, colon histopathology, and the levels of toxins in the feces were measured. The expressions of TNF alpha, MCP-1, NF-kappa B p65, and phospho-NF-kappa B p65 in the colon were presented by immunohistochemistry. The survival rate of QPYF group (93.75%) was higher than that of model control group (65%). The mice treated with QPYF had a lower weight loss and disease activity index, compared to the mice with placebo. A significantly lower level of histopathology scores, toxins in the feces, and TNF.., MCP-1, NF-kappa B p65, and phospho-NF-kappa B p65 were detected for QPYF-treated mice. Traditional Chinese medicine QPYF showed a good preventive effect for CDAD in a mouse model.
第一作者单位：[1]Department of Pediatrics, China-Japan Friendship Hospital, Beijing, China
通讯作者：
推荐引用方式(GB/T 7714)：
Guo Ya-Nan,Wang Jun,Zhang Hao-Jun,et al.Traditional Chinese Medicine QPYF as Preventive Treatment for Clostridium difficile Associated Diarrhea in a Mouse Model[J].EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE.2016,2016:doi:10.1155/2016/3759819.
APA：
Guo Ya-Nan,Wang Jun,Zhang Hao-Jun,Jia Hong-Bing,Li Ping&Liu Xin-Zhu.(2016).Traditional Chinese Medicine QPYF as Preventive Treatment for Clostridium difficile Associated Diarrhea in a Mouse Model.EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE,2016,
MLA：
Guo Ya-Nan,et al."Traditional Chinese Medicine QPYF as Preventive Treatment for Clostridium difficile Associated Diarrhea in a Mouse Model".EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE 2016.(2016)
