单位：[1]Minist Educ, Key Lab Chinese Internal Med, Beijing 100700, Peoples R China[2]Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China[3]China Acad Chinese Med Sci, Southern Dist Guanganmen Hosp, Beijing 102618, Peoples R China[4]China Acad Chinese Med Sci, Guanganmen Hosp, Beijing 100053, Peoples R China[5]China Acad Chinese Med Sci, Inst Informat Tradit Chinese Med, Beijing 100700, Peoples R China[6]China Japan Friendship Hosp, Dept Prevent Hlth Care, Beijing 10029, Peoples R China
Qingkailing (QKL) is a well-known composite extract used in traditional Chinese medicine. This extract has been extensively administered to treat the acute phase of cerebrovascular disease. Our previous experiments confirmed that QKL exerts an inhibitory effect on cerebral ischemia-induced inflammatory responses. However, whether QKL suppresses the activation of microglia, the primary resident immune cells in the brain, has yet to be determined. In this study, BV2 microglial cells were used to validate the protective effects of QKL treatment following ischemia-reperfusion injury simulated via hypoxia/reoxygenation in vitro. Under these conditions, high expression levels of ROS, COX-2, iNOS, and p-p38 protein were detected. Following ischemia/reperfusion injury, QKL significantly increased the activity of BV2 cells to approximately the basal level by modulating microglial activation via inhibition of inflammatory factors, including TNF-alpha, COX-2, iNOS, and p-p38. However, QKL treatment also displayed dose-dependent differences in its inhibitory effects on p38 phosphorylation and inflammatory factor expression.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81173229]; independent subject of Beijing University of Chinese Medicine [2011-JYB ZZJS-001]
第一作者单位：[1]Minist Educ, Key Lab Chinese Internal Med, Beijing 100700, Peoples R China[2]Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
通讯作者：
通讯机构：[1]Minist Educ, Key Lab Chinese Internal Med, Beijing 100700, Peoples R China[2]Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
推荐引用方式(GB/T 7714)：
Mana Lulu,Wang Shan,Zhu Haiyan,et al.Qingkailing Suppresses the Activation of BV2 Microglial Cells by Inhibiting Hypoxia/Reoxygenation-Induced Inflammatory Responses[J].EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE.2014,2014:doi:10.1155/2014/696218.
APA：
Mana, Lulu,Wang, Shan,Zhu, Haiyan,Xing, Yanwei,Lou, Lixia...&Gao, Yonghong.(2014).Qingkailing Suppresses the Activation of BV2 Microglial Cells by Inhibiting Hypoxia/Reoxygenation-Induced Inflammatory Responses.EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE,2014,
MLA：
Mana, Lulu,et al."Qingkailing Suppresses the Activation of BV2 Microglial Cells by Inhibiting Hypoxia/Reoxygenation-Induced Inflammatory Responses".EVIDENCE-BASED COMPLEMENTARY and ALTERNATIVE MEDICINE 2014.(2014)
