Relaxin is a pleiotropic hormone owing endogenous antifibrosis effect on numerous organs. We demonstrated relaxin's inhibitive effect on cardiac fibrosis previously. The aim of this study was to investigate the role of protein kinase C (PKC) beta(2) in relaxin's action under high glucose conditions. Cardiac fibroblasts (CFs) were isolated, exposed to high glucose and incubated with recombinant human relaxin (rhRLX). Western blot analysis revealed a relaxin-mediated decrease in total expression and translocation of PKC beta(2), showing downregulation of PKC beta(2) is involved in relaxin's action. Blocking PKC beta(2) pathway with ruboxistaurin accelerated rhRLX-mediated inhibition in both proliferation of CFs and deposition of collagen. In conclusion, relaxin can inhibit high glucose-associated cardiac fibrosis partly through PKC beta(2) pathway. Further work should be done to fully understand intracellular mechanisms of relaxin's action to accelerate its clinical use.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81100169]
第一作者单位：[1]Capital Med Univ, Beijing Friendship Hosp, Dept Cardiol, Beijing 100050, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Friendship Hosp, Dept Cardiol, Beijing 100050, Peoples R China[*1]Capital Med Univ, Beijing Friendship Hosp, Dept Cardiol, 95 Yongan Rd, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Su W.,Wang P.,Chen H.,et al.Role of protein kinase C beta(2) in relaxin-mediated inhibition of cardiac fibrosis[J].JOURNAL of ENDOCRINOLOGICAL INVESTIGATION.2014,37(6):559-564.doi:10.1007/s40618-014-0068-7.
APA：
Su, W.,Wang, P.,Chen, H.&Li, H..(2014).Role of protein kinase C beta(2) in relaxin-mediated inhibition of cardiac fibrosis.JOURNAL of ENDOCRINOLOGICAL INVESTIGATION,37,(6)
MLA：
Su, W.,et al."Role of protein kinase C beta(2) in relaxin-mediated inhibition of cardiac fibrosis".JOURNAL of ENDOCRINOLOGICAL INVESTIGATION 37..6(2014):559-564
