单位：[1]Chinese Acad Med Sci, Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China[2]Peking Union Med Coll, Beijing 100021, Peoples R China[3]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing 100029, Peoples R China[4]Univ Manitoba, Fac Pharm, Winnipeg, MB R3E 0T5, Canada
DNase I is an endonuclease responsible to destruction of chromatin during apoptosis. However, its role in diabetes is still unclear. With blood samples from our previous study related to type 2 diabetes, we examined the DNase I activity in the serum of these patients and the role of DNase I in the injury of pancreas was further investigated in rats and INS-1 cells. Serum and pancreatic tissues from human and rats were used for the study. Insulin resistance and diabetes were induced by high fat diet and STZ injection, respectively. DNase I activity was determined by radial enzyme-diffusion method. Expressions of DNase I and caspase-3 in pancreas were determined in rat pancreatic tissues and INS-1 cells. Apoptosis of INS-1 cells was determined by both TUNEL assay and Flow Cytometry. There was a significant elevation of DNase I activity in serum of patients with type 2 diabetes and rats with STZ injection. Moreover, increase in DNase I expression was observed in the pancreas of diabetic person and rats. Furthermore, high glucose induced both DNase I and caspase-3 expression and at the same time increased apoptosis rate of INS-1 cells. In conclusion, elevated DNase I in diabetes may be related to pancreatic injury and could be one of the causes that induce diabetes.
基金：
National Natural Scientific Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81173422, 81130066]; program of China-International ST Cooperation [2011DFA31860]
第一作者单位：[1]Chinese Acad Med Sci, Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China[2]Peking Union Med Coll, Beijing 100021, Peoples R China[3]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing 100029, Peoples R China
通讯作者：
通讯机构：[1]Chinese Acad Med Sci, Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China[2]Peking Union Med Coll, Beijing 100021, Peoples R China[3]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing 100029, Peoples R China
推荐引用方式(GB/T 7714)：
Zhu Bin,Gong Yuewen,Chen Pengmin,et al.Increased DNase I activity in diabetes might be associated with injury of pancreas[J].MOLECULAR and CELLULAR BIOCHEMISTRY.2014,393(1-2):23-32.doi:10.1007/s11010-014-2043-1.
APA：
Zhu, Bin,Gong, Yuewen,Chen, Pengmin,Zhang, Haojun,Zhao, Tingting&Li, Ping.(2014).Increased DNase I activity in diabetes might be associated with injury of pancreas.MOLECULAR and CELLULAR BIOCHEMISTRY,393,(1-2)
MLA：
Zhu, Bin,et al."Increased DNase I activity in diabetes might be associated with injury of pancreas".MOLECULAR and CELLULAR BIOCHEMISTRY 393..1-2(2014):23-32
