The present study investigates the effect of Chang Run Tong (CRT) on the biomechanical and morphometrical remodeling of colon and rectum in streptozotocin-induced diabetic rats. The colonic and rectal segments were obtained from diabetic (DM), CRT-treated diabetic (Ti, high dosage: 50 g/kg/day, T2, low dosage: 25 g/kg/day) and normal (Con) rats. The experimental period was 60 d. The blood glucose level and body weight were measured. The circumferential length, wall thickness, and opening angle were measured from the segments in the no-load and zero-stress states. The residual strain was computed from the morphometry data. The step-wise distension was done on the colonic segment (from 0 to 20 cmH(2)O). The circumferential and longitudinal stresses and strains were computed. The blood glucose level was significantly higher and the body weight was significantly lower in the DM, Ti, and T2 groups compared to those in the Con group (p < 0.01, p < 0.001). The glucose level did not differ among the DM, Ti, and T2 groups. The wet weight per unit length to body weight ratio, wall thickness, cross-sectional wall area, opening angle, and absolute value of residual strain of colonic and rectal segments in the DM group were significantly higher than those in the Con group (p < 0.05 and p < 0.01), and those in the Ti group, but not those in the T2 group, were significantly lower than those in the DM group (p < 0.05, p < 0.01). Furthermore, the circumferential and longitudinal stiffness of the colonic wall in the DM group was higher than those in the Con group. Ti, but not T2, treatment could significantly decrease the colonic wall stiffness in both directions (p < 0.01). CRT (high dose) treatment could partly restore the morphometric and biomechanical remodeling of the lower gastrointestinal tract in diabetic rats.