单位：[1]Chinese Peoples Liberat Army Gen Hosp, Dept Hematol, Beijing 100853, Peoples R China[2]Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Dept Oncol, Beijing 100048, Peoples R China[3]Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Dept Pharm, Beijing 100048, Peoples R China[4]China Japan Friendship Hosp, Dept Hematol, Beijing 100029, Peoples R China[5]Chinese Peoples Liberat Army, Hosp 535, Huaihua 418000, Hunan, Peoples R China
Background. Cancer/testis antigens (CTAs) are ideal targets for cancer immunotherapy in virtue of their restricted expression profile in normal tissues. However, CTA-targeted immunotherapy has been rather disappointing clinical setting for CTAs are downregulated by cytosine-phosphate-guanosine (CpG) methylation in their promoter regions, so that tumor cells have low immunogenicity. Methods. We reinduced mouse CTA P1A through demethylation process and generated P1A-specific cytotoxic lymphocytes (CTLs) by immunizing BALB/c (H-2(d)) mice with dendritic cells pulsed with a P1A-specific peptide and CpG oligodeoxynucleotide (ODN) immune adjuvant. Results. We found that demethylation and CpG ODN immune adjuvant stimulation facilitated DC maturation and enhanced the allogenic capacity of P1A-specific CTLs against target cells both in vitro and in vivo. Conclusions. Our results suggested that CTA induction and immune adjuvant stimulation is a feasible strategy in cancer immunotherapy.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30572108, 30971297]; National 973 Project of ChinaNational Basic Research Program of China [2005CB522400]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2012]版：
无
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2011]版：
无
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Chinese Peoples Liberat Army Gen Hosp, Dept Hematol, Beijing 100853, Peoples R China[2]Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Dept Oncol, Beijing 100048, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Sun Jun-Zhong,Gao Lei,Gao Li,et al.Demethylation of Cancer/Testis Antigens and CpG ODN Stimulation Enhance Dendritic Cell and Cytotoxic T Lymphocyte Function in a Mouse Mammary Model[J].BIOMED RESEARCH INTERNATIONAL.2013,2013:doi:10.1155/2013/196894.
APA：
Sun, Jun-Zhong,Gao, Lei,Gao, Li,Wang, Wei,Du, Nan...&Yu, Li.(2013).Demethylation of Cancer/Testis Antigens and CpG ODN Stimulation Enhance Dendritic Cell and Cytotoxic T Lymphocyte Function in a Mouse Mammary Model.BIOMED RESEARCH INTERNATIONAL,2013,
MLA：
Sun, Jun-Zhong,et al."Demethylation of Cancer/Testis Antigens and CpG ODN Stimulation Enhance Dendritic Cell and Cytotoxic T Lymphocyte Function in a Mouse Mammary Model".BIOMED RESEARCH INTERNATIONAL 2013.(2013)
