The supersensitive detection of clenbuterol was achieved by a method of combining molecularly imprinted polymer with surface plasmon resonance in this article. The detection limit of clenbuterol was I x 10(-15) mol L-I, and the changes in resonance angle (Delta theta) had a good linear relationship with the logarithm of clenbuterol concentration in the range from 1 x 10(-15) - 1 x 10(-7) mol L-1. Selectivity experiment showed that no Delta theta was observed in response to salbutamol until the concentration of 1 x 10(-5) mol L-1.