Programmed cell death, or apoptosis, is one of the fates of the medial edge epithelium (MEE) during palatal fusion. Transforming growth factor beta (Tgf-beta) signaling (such as Tgf-beta 3) is required for the disappearance of the MEE, but the relationship between Tgf-beta 3 and apoptosis remains unclear. Here we show that the Fas ligand (FasL)-Fas-Caspase extrinsic apoptosis pathway functions during palatal fusion in wild-type mice, but is not detectable in mice lacking Tgf-beta 3 (Tgf-beta 3(-/-)) or Tgf beta r2 in the MEE (K14-Cre; Tgfbr2(fl/fl)). Inhibition of the FasL-Fas system results in persistence of the midline epithelial seam (MES) and inhibition of caspase activity during palatal organ culture. Moreover, ectopic FasL protein induces apoptosis in MES of K14-Cre; Tgfbr2(fl/fl) mice. Thus, we conclude that the FasL-Fas-caspase extrinsic apoptosis pathway is regulated by the Tgf-beta 3 signaling cascade and is essential for palatal fusion during craniofacial development.
基金：
NIDCR, NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [R37 DE012711, R01 DE014078]; Beijing New Star Program [2007B54]; NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [R01DE014078, R37DE012711] Funding Source: NIH RePORTER
第一作者单位：[1]Univ So Calif, Ctr Craniofacial Mol Biol, Sch Dent, Los Angeles, CA 90033 USA[2]Capital Med Univ, Beijing Friendship Hosp, Dept Stomatol, Beijing 100050, Peoples R China
通讯作者：
通讯机构：[1]Univ So Calif, Ctr Craniofacial Mol Biol, Sch Dent, Los Angeles, CA 90033 USA[*1]Univ So Calif, Ctr Craniofacial Mol Biol, Sch Dent, 2250 Alcazar St,CSA 103, Los Angeles, CA 90033 USA
推荐引用方式(GB/T 7714)：
Huang X.,Yokota T.,Iwata J.,et al.Tgf-beta-mediated FasL-Fas-Caspase Pathway Is Crucial during Palatogenesis[J].JOURNAL of DENTAL RESEARCH.2011,90(8):981-987.doi:10.1177/0022034511408613.
APA：
Huang, X.,Yokota, T.,Iwata, J.&Chai, Y..(2011).Tgf-beta-mediated FasL-Fas-Caspase Pathway Is Crucial during Palatogenesis.JOURNAL of DENTAL RESEARCH,90,(8)
MLA：
Huang, X.,et al."Tgf-beta-mediated FasL-Fas-Caspase Pathway Is Crucial during Palatogenesis".JOURNAL of DENTAL RESEARCH 90..8(2011):981-987
医学