The purpose of this study was to investigate the mechanism of expression of matrix metalloproteinase-13 (MMP-13) induced by nitric oxide (NO). Human chondrocytes (HCs) were stimulated with a NO donor (MAHMA-NONOate), then mitogen-activated protein kinases' (MAPKs) and nuclear factor kappa B' (NF-kappa B) activations and MMP-13' expression were assayed by Western blot analysis. Additionally, the intracellular signalling of NO was investigated using the inhibitors of MAPKs and NF-kappa B. NO-induced MMP-13 expression was not suppressed by extracellular signal-regulated kinase (ERK) inhibitor (PD98059) or inhibitors of p38 kinase (SB203580), but was inhibited by a c-jun terminal kinase (JNK) inhibitor (SP600125) and inhibitors of NF-kappa B (SN-50). Additionally, SP600125 treatment reduced NF-kappa B activation, but SN-50 treatment did not significantly affect JNK activation. These results suggest that NO induces MMP-13 expression by JNK and NF-kappa B activation in HCs.
第一作者单位：[1]Capital Med Univ, Dept Orthopaed, Beijing Friendship Hosp, Beijing 100050, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Lin,Guo Ai,Gu Jun-Chao.c-Jun N-terminal kinase and nuclear factor kappa B mediate nitric oxide-induced expression of matrix metalloproteinase-13[J].INTERNATIONAL ORTHOPAEDICS.2011,35(8):1261-1266.doi:10.1007/s00264-010-1056-y.
APA：
Yang, Lin,Guo, Ai&Gu, Jun-Chao.(2011).c-Jun N-terminal kinase and nuclear factor kappa B mediate nitric oxide-induced expression of matrix metalloproteinase-13.INTERNATIONAL ORTHOPAEDICS,35,(8)
MLA：
Yang, Lin,et al."c-Jun N-terminal kinase and nuclear factor kappa B mediate nitric oxide-induced expression of matrix metalloproteinase-13".INTERNATIONAL ORTHOPAEDICS 35..8(2011):1261-1266
