单位：[1]China Japan Friendship Hosp, Dept Endocrinol, Beijing 100029, Peoples R China[2]Tsinghua Univ, Peking Union Med Coll, Grad Sch, Beijing 100084, Peoples R China[3]Boston Univ, Sch Med, Dept Med, Vasc Biol Unit, Boston, MA 02118 USA
The aim of the study is to investigate the possible mechanism of oxidative stress in the high free fatty acids (FFAs)-induced hypertension. Male Sprague-Dawley rat models were established and classified into three groups, namely the control group (NC group), the FFA group, and the N-acetylcysteine (NAC) group. Blood pressure (BP) was recorded. An organ chamber experiment was performed to determine endothelium-dependent/-independent vasodilation (EDV/EIV). Reactive oxygen species (ROS), nitrotyrosine, reduced glutathione hormone (GSH) and NO2-/NO3- levels were measured in plasma. Endothelial nitric oxide synthase (eNOS) mRNA expression in endothelial cells was evaluated by real-time PCR. The following results were observed: (1) In the FFA group, BP increased after 4 h infusion of Intralipid+heparin. In the NAC group, systolic and diastolic BP remained the same. (2) In the FFA group, the aortic rings tended to show impaired EDV in response to acetylcholine (ACh). There was no difference of EDV response in the NAC and NC groups. (3) In the FFA group, NO2-/NO3- levels were significantly reduced, and eNOS mRNA expression and activity were significantly decreased compared with the NC group. NAC administration increased eNOS mRNA expression and activity. (4) ROS and nitrotyrosine concentrations in the FIFA group were higher than in the NC group, and GSH concentrations in the FFA group were lower than in the NC group. Elevated FFAs can induce elevated BP, potentially through FFA-induced impairment of EDV resulting from decreased eNOS mRNA expression and activity. Oxidative stress may also play an important role in potential mechanisms of this high FFA-induced elevated BR
基金：
Clinical Research Institute of China-Japan Friendship Hospital
第一作者单位：[1]China Japan Friendship Hosp, Dept Endocrinol, Beijing 100029, Peoples R China[2]Tsinghua Univ, Peking Union Med Coll, Grad Sch, Beijing 100084, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Hui,Li Hongliang,Hou Zhiqiang,et al.Role of oxidative stress in elevated blood pressure induced by high free fatty acids[J].HYPERTENSION RESEARCH.2009,32(2):152-158.doi:10.1038/hr.2008.35.
APA：
Wang, Hui,Li, Hongliang,Hou, Zhiqiang,Pan, Lin,Shen, Xiaoxia&Li, Guangwei.(2009).Role of oxidative stress in elevated blood pressure induced by high free fatty acids.HYPERTENSION RESEARCH,32,(2)
MLA：
Wang, Hui,et al."Role of oxidative stress in elevated blood pressure induced by high free fatty acids".HYPERTENSION RESEARCH 32..2(2009):152-158
