单位：[1]Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, Beijing 100050, Peoples R China临床科室国家中心肝病分中心首都医科大学附属北京友谊医院[2]Univ Arkansas Med Sci, Dept Internal Med, Gene Therapy Ctr, Little Rock, AR 72205 USA
Rep78, the rep gene product of adeno-associated virus (AAV), has been shown to inhibit the replication of several DNA viruses. This study investigated the effects of Rep78 on replication of Hepatitis B virus (HBV) and possible mechanisms of inhibition. We have shown that HBV DNA replication and secretion of HBsAg and HBeAg in HepG2 2.2.15 cells were inhibited by Rep78. We have also demonstrated, using in vitro transcription and luciferase assay, that Rep78 binds to the HBV core promoter (HBV CP) and inhibits HBV CP activity. Furthermore, after Rep78 and HBV core protein expression plasmids were co-transfected into HepG2 cells, the expression of HBV core protein was inhibited significantly. These results suggest that Rep78 can inhibit the replication of HBV, correlating strongly with suppression of HBV CP activity. (C) 2009 Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30500425]; National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2006AA02A410]; Major State Basic Research Development ProgramNational Basic Research Program of China [2007CB512802]
第一作者单位：[1]Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, Beijing 100050, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, Beijing 100050, Peoples R China[*1]Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, 95 YongAn Rd, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Liu Tianhui,Cong Min,Wang Ping,et al.Adeno-associated virus Rep78 protein inhibits Hepatitis B virus replication through regulation of the HBV core promoter[J].BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS.2009,385(1):106-111.doi:10.1016/j.bbrc.2009.04.132.
APA：
Liu, Tianhui,Cong, Min,Wang, Ping,Jia, Jidong,Liu, Yong...&You, Hong.(2009).Adeno-associated virus Rep78 protein inhibits Hepatitis B virus replication through regulation of the HBV core promoter.BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS,385,(1)
MLA：
Liu, Tianhui,et al."Adeno-associated virus Rep78 protein inhibits Hepatitis B virus replication through regulation of the HBV core promoter".BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS 385..1(2009):106-111
