A sensitive and specific method was developed for simultaneous determination of 21 compounds related to the diabetic nephropathy (DN) in a single analysis using high-performance liquid chromatography coupled to ultraviolet and tandem mass spectrometry (HPLC-UV/MS/MS) in human plasma. With retention times and MS/MS for peak identification, both UV and MS detectors were used for quantification. Calibration curves suitable for the analysis of plasma were linear (r(2) > 0.998) with limits of detection (LOD) from 10 to 1000 ng/mL. Intraday relative standard deviation (R.S.D.) and interday R.S.D. were both lower than 15%. With the case and control study, we found five potential biomarkers of DN, including adenosine, inosine, uric acid, xanthine and creatinine. (C) 2009 Elsevier B.V. All rights reserved.
基金：
Sino-Japanese Friendship Hospital (Beijing, China); National Basic Research Development Program of China (973 Program)National Basic Research Program of China [2005CB523503]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [90709045]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2008]版：
大类|3 区医学
小类|2 区分析化学3 区生化研究方法
最新[2025]版：
大类|3 区医学
小类|3 区生化研究方法3 区分析化学
JCR分区：
出版当年[2007]版：
Q1CHEMISTRY, ANALYTICALQ2BIOCHEMICAL RESEARCH METHODS
最新[2024]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICAL
第一作者单位：[2]E China Univ Sci & Technol, Sch Pharmaceut, Shanghai 200237, Peoples R China
通讯作者：
通讯机构：[1]Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China[2]E China Univ Sci & Technol, Sch Pharmaceut, Shanghai 200237, Peoples R China
推荐引用方式(GB/T 7714)：
Xia Jian-Fei,Liang Qiong-Lin,Liang Xiao-Ping,et al.Ultraviolet and tandem mass spectrometry for simultaneous quantification of 21 pivotal metabolites in plasma from patients with diabetic nephropathy[J].JOURNAL of CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES in the BIOMEDICAL and LIFE SCIENCES.2009,877(20-21):1930-1936.doi:10.1016/j.jchromb.2009.05.047.
APA：
Xia, Jian-Fei,Liang, Qiong-Lin,Liang, Xiao-Ping,Wang, Yi-Ming,Hu, Ping...&Luo, Guo-An.(2009).Ultraviolet and tandem mass spectrometry for simultaneous quantification of 21 pivotal metabolites in plasma from patients with diabetic nephropathy.JOURNAL of CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES in the BIOMEDICAL and LIFE SCIENCES,877,(20-21)
MLA：
Xia, Jian-Fei,et al."Ultraviolet and tandem mass spectrometry for simultaneous quantification of 21 pivotal metabolites in plasma from patients with diabetic nephropathy".JOURNAL of CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES in the BIOMEDICAL and LIFE SCIENCES 877..20-21(2009):1930-1936
