单位：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Dept Mol Genet, Beijing 100850, Peoples R China[2]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China[3]Peking Univ, Dept Biol, Beijing 100871, Peoples R China
The anti-morphine peptide from canine brain, which is homologous to alpha subunit of hemoglobin of canine, has been isolated and characterized. The canine brain was homogenized and extracted in 0.1 hi acetic acid The supernatant was collected, and purified by chromatography of Sephadex G-50 and ion-exchange and RP-HPLC. A polypeptide was then obtained, which is termed as AMPS. It shows prominent anti-morphine analgesic activity. AMP5 has an apparent molecular mass of 10.23kD. The isoelectric point was estimated to be around pH 6.7 by IEF. The amino acid composition of AMPS and sequence of the N-terminal 50 amino acid residues was determined.
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外文
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第一作者：
第一作者单位：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Dept Mol Genet, Beijing 100850, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Chen SJ,Chen YZ,Wang JY.Isolation and characterization of anti morphine analgesic peptide from canine brain[J].BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL.1997,43(3):583-590.
APA：
Chen, SJ,Chen, YZ&Wang, JY.(1997).Isolation and characterization of anti morphine analgesic peptide from canine brain.BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL,43,(3)
MLA：
Chen, SJ,et al."Isolation and characterization of anti morphine analgesic peptide from canine brain".BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL 43..3(1997):583-590
