The microstructure, composition and the micromechanical properties across the thickness of femoral mid-diaphyses from 14 to 26 week human fetuses have been investigated, Scanning electron microscopy and transmission electron microscopy were employed to examine structural changes with maturation, The fetal bones consist of layers of woven bone. From young to old fetuses and from outer to inner bone layers, the collagen fibrils become more cross-linked, densely packed and change from disordered to an ordered arrangement. The collagen fibril bundles are also more preferentially oriented and change from a chiefly circumferential to longitudinal direction, The sizes of the apatite crystals also increase with age, The Ca/P ratio remains constant around 1.55 for all the bone layers except the outmost layer which is lower than 1.2, An nano-indenter was used to evaluate the microhardness and elastic modulus of each bone layer, The increase of microhardness and elastic modulus correlates with the maturation of bone, The mechanical properties of the mid-diaphyses of human fetal femurs are anisotropic, which is due to the preferential orientation of collagen fibrils.