单位：[1]Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing, China,医技科室药学部首都医科大学附属北京友谊医院[2]Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, China,[3]Interventional Department, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China,内蒙古科技大学包头医学院[4]Department of Technology, Stem Cell Medicine Engineering & Technology Research Center of Inner Mongolia, Huhhot, China,[5]Department of Blood, The People’s Hospital of Xing’an League, Ulanhot, China
The usage of animal serum may ultimately prevent the application ofex vivocultured mesenchymal stromal cells (MSCs) in a clinical setting due to safety concerns and batch-to-batch variability. Increasing regulatory pressure to limit use of animal serum has been issued and serum-free, xeno-free, and chemically defined media (S&XFM-CD) is encouraged to replace serum-containing media (SCM) in the stem cell preparation process. We previously developed a S&XFM-CD for the expansion of umbilical cord-derived MSCs (UCMSCs). Different culture conditions affect the function of MSCs, which may further affect the therapeutic efficiency and mechanisms of action. In this study, we compared the therapeutic effect and mechanism of UCMSCs in S&XFM-CD (UCMSCS&XFM-CD) in experimental colitis with those in SCM (UCMSCSCM). UCMSC(S&XFM-CD)exhibited better therapeutic effects than UCMSC(SCM)by body weight, disease activity index, and histological colitis score. UCMSC(S&XFM-CD)or UCMSC(SCM)migrated to the inflammation site of injured colon, but exhibited low levels of recruitment and persistence. Systemic depletion of endogenous macrophages impaired the therapeutic effects of UCMSC(SCM)and UCMSCS&XFM-CD. Furthermore, UCMSC(S&XFM-CD)more markedly promoted intestinal macrophage polarisation from M1 to M2 phenotype to produce higher levels of IL-10 and lower levels of TNF-alpha in colon tissue than UCMSCSCM, while a higher level of IL-4 was produced in UCMSCSCM-treated group. UCMSC(S&XFM-CD)cocultured with RAW264.7 cells in a transwell system promoted the release of TSG-6 and IL-6, whereas UCMSC(SCM)increased PGE(2)levels. Taken together, we demonstrated that UCMSCs in S&XFM-CD exhibited improved therapeutic effects with altered cytokine secretion in an experimental acute colitis model.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81960252, 81860157]; Natural Science Foundation of Inner Mongolia [2019MS08047]
第一作者单位：[1]Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing, China,[2]Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, China,[3]Interventional Department, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China,[4]Department of Technology, Stem Cell Medicine Engineering & Technology Research Center of Inner Mongolia, Huhhot, China,
通讯作者：
推荐引用方式(GB/T 7714)：
Wu X,Wu D,Mu Y,et al.Serum-Free Medium Enhances the Therapeutic Effects of Umbilical Cord Mesenchymal Stromal Cells on a Murine Model for Acute Colitis[J].FRONTIERS in BIOENGINEERING and BIOTECHNOLOGY.2020,8:doi:10.3389/fbioe.2020.00586.
APA：
Wu, X,Wu, D,Mu, Y,Zhao, Y&Ma, Z.(2020).Serum-Free Medium Enhances the Therapeutic Effects of Umbilical Cord Mesenchymal Stromal Cells on a Murine Model for Acute Colitis.FRONTIERS in BIOENGINEERING and BIOTECHNOLOGY,8,
MLA：
Wu, X,et al."Serum-Free Medium Enhances the Therapeutic Effects of Umbilical Cord Mesenchymal Stromal Cells on a Murine Model for Acute Colitis".FRONTIERS in BIOENGINEERING and BIOTECHNOLOGY 8.(2020)
