单位：[1]Liver Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.临床科室国家中心肝病分中心首都医科大学附属北京友谊医院
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摘要：
To clone the partial cDNA sequence of rat connective tissue growth factor (CTGF) and investigate the mRNA expression of CTGF and transforming growth factor-beta 1 (TGF-beta 1) in rat experimental liver fibrosis.A 430-base pair sequence of rat CTGF Cdna was cloned by reverse transcription-polymerase chain reaction. Sixteen female Wistar rats were allocated into bile duct occlusion group (n=8) and sham-operated group (n=8). The liver tissue mRNA levels of CTGF and TGF-beta 1 were measured by multiprobe ribonuclease protection assay (RPA).The cloned partial cDNA sequence of rat CTGF was 95% homology (at nucleic acid level) with the mouse counterpart. In the liver of rats with biliary fibrosis mRNA levels of TGF-beta 1 and CTGF were 4 and 7 times as high as in those of sham-operated rats, respectively.The mRNA expression of CTGF is remarkably upregulated in rat liver fibrogenesis.
语种：
中文
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第一作者：
第一作者单位：[1]Liver Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
推荐引用方式(GB/T 7714)：
Jia J,Bauer M,Boigk G.Cloning of partial cDNA of rat connective tissue growth factor and expression of its mRNA in experimental liver fibrosis[J].Chinese Journal of Hepatology.2000,8(2):102-4.
