Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China
单位：[1]Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, CapitalMedical University, Beijing 100050, China首都医科大学附属北京友谊医院[2]Beijing Key Laboratory forResearch on Prevention and Treatment of Tropical Diseases, Beijing 100050,China[3]Department of Gastroenterology, Beijing Children’s Hospital, CapitalMedical University, Beijing 100045, China首都医科大学附属北京儿童医院[4]Outpatient Department, BeijingFriendship Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属北京友谊医院[5]NIHRHealth Protection Research Unit for Emerging and Zoonotic Infections,University of Liverpool, Liverpool L69 7BE, UK[6]Tropical and InfectiousDisease Unit, Royal Liverpool University Hospital (member of LiverpoolHealth Partners), Liverpool L7 8XP, UK[7]State Key Laboratory of AgriculturalMicrobiology, College of Veterinary Medicine, Huazhong AgriculturalUniversity, Wuhan 430070, Hubei, China[8]Beijing Key Laboratory of PediatricRespiratory Infection diseases, Key Laboratory of Major Diseases in Children,Ministry of Education, National Clinical Research Center for Respiratory Diseases, Research Unit of Critical Infection in Children, Chinese Academy ofMedical Sciences, 2019RU016, Laboratory of Infection and Virology, BeijingPediatric Research Institute, Beijing Children’s Hospital, Capital MedicalUniversity, National Center for Children’s Health, Beijing 100045, China首都医科大学附属北京儿童医院[9]Department of Internal Medicine, Beijing Obstetrics and GynecologyHospital, Capital Medical University, Beijing 100026, China
Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after sufficient vaccine coverage. The live-attenuated Japanese encephalitis virus (JEV) vaccine SA14-14-2 is believed to have strongly contribute to this decrease. Another vaccine that seems to have decreased in importance is an inactivated vaccine based on the JEV P3 strain, which is considered to be modifiable, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by the Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding the premembrane (prM) and envelope (E) proteins of the P3 strain was assessed in BALB/c mice. TheprM/Egenes of the JEV P3 strain were subcloned into the vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized into BALB/c mice, and high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes were secreted upon stimulation with JEV antigens. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in the mice. Conclusions The DNA vaccine pV-JP3ME based on the JEV P3 strain in this study can induce specific humoral immune and cytokine responses and provide complete protection against JEV in mice.
基金：
Cultivation Fund Project of the National Natural Science Foundation at Beijing Children's Hospital, Capital Medical University [GPQN201909]; Research Foundation of Capital Medical University [PYZ2018052]; Startup Fund of Beijing Friendship Hospital, Capital Medical University [Yyqdkt2016-24]; Wellcome clinical career development fellow [205228/Z/16/Z]
第一作者单位：[1]Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, CapitalMedical University, Beijing 100050, China[2]Beijing Key Laboratory forResearch on Prevention and Treatment of Tropical Diseases, Beijing 100050,China
通讯作者：
推荐引用方式(GB/T 7714)：
Zheng Xiaoyan,Yu Xiaozheng,Wang Yan,et al.Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China[J].VIROLOGY JOURNAL.2020,17(1):doi:10.1186/s12985-020-01400-3.
APA：
Zheng Xiaoyan,Yu Xiaozheng,Wang Yan,Turtle Lance,Cui Min...&Yin Chenghong.(2020).Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China.VIROLOGY JOURNAL,17,(1)
MLA：
Zheng Xiaoyan,et al."Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China".VIROLOGY JOURNAL 17..1(2020)
