单位：[1]Department of Neurology, China-Japan Friendship Hospital, Beijing 100029 China[2]State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China[3]Genmedicn Biopharma, Beijing 100176 China
We have generated PUMCi001-A, an induced pluripotent stem cells (iPSC) line from dermal fibroblasts of a 13-year-old male Krabbe disease patient with two hemizygous (461C > A and 1244G > A) mutations in Galactocerebrosidase (GALC) gene using a Sendai viral delivery of OCT4, SOX2, KLF4, and c-MYC. The PUMCi001-A iPSC line carried the GALC mutations, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited a normal karyotype and differentiation capacity into three germ layers.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81701131]; CAMS Initiative for Innovative Medicine [2016-I2M-3-002]
第一作者单位：[1]Department of Neurology, China-Japan Friendship Hospital, Beijing 100029 China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Wei,Lv Ya-feng,Zhang Yan-jun,et al.Generation of a human induced pluripotent stem cell line PUMCi001-A from a patient with Krabbe disease[J].STEM CELL RESEARCH.2020,48:doi:10.1016/j.scr.2020.101937.
APA：
Wang, Wei,Lv, Ya-feng,Zhang, Yan-jun,Dong, Wen-ji&Zhang, Ye.(2020).Generation of a human induced pluripotent stem cell line PUMCi001-A from a patient with Krabbe disease.STEM CELL RESEARCH,48,
MLA：
Wang, Wei,et al."Generation of a human induced pluripotent stem cell line PUMCi001-A from a patient with Krabbe disease".STEM CELL RESEARCH 48.(2020)
