单位：[1]Bioengineering, college of Biological Engineering and Food, Hubei University of Technology, P.R. China.[2]Beijing Institute of Biotechnology, Beijing, P.R. China.[3]Clinical Laboratory Center, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China.医技科室检验科检验科首都医科大学附属北京友谊医院[4]Department of Gastroenterology and Hepatology, the Fifth Medical Center of Chinese PLA General Hospital, Beijing, P.R. China[5]Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329),[6]Beijing Hotgen Biotechnology Inc., Beijing 100071, P. R. China[7]These authors contributed equally to this work
The Gram-negative bacterial pathogenYersiniadelivers six effector proteins into the host cells to block the host innate immune response. One of the effectors, YopT, is a potent cysteine protease that causes the disruption of the actin cytoskeleton to inhibit phagocytosis of the pathogen; however, its molecular mechanism and relevance to pathogenesis need further investigation. In this report, we show that RIG-I is a novel target of the YopT protein. Remarkably, YopT interacts with RIG-I and inhibits rat liver homogenate-mediated nuclear factor-kappa B and interferon regulatory factor-3 activation. Further studies revealed a YopT-dependent increase in the K48-polymerized ubiquitination of RIG-I. These findings suggest that YopT negatively regulates RIG-I-mediated cellular antibacterial response by targeting RIG-I.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81671973, 31670761, 31872715, 81773205]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7182122, 5182029, 5182030]
第一作者单位：[1]Bioengineering, college of Biological Engineering and Food, Hubei University of Technology, P.R. China.[2]Beijing Institute of Biotechnology, Beijing, P.R. China.[7]These authors contributed equally to this work
通讯作者：
通讯机构：[1]Bioengineering, college of Biological Engineering and Food, Hubei University of Technology, P.R. China.[7]These authors contributed equally to this work
推荐引用方式(GB/T 7714)：
Li Dongyu,Wang Haoyong,Zhang Pingping,et al.Yersinia YopT inhibits RLH-mediated NF-kappa B and IRF3 signal transduction[J].MICROBIOLOGY and IMMUNOLOGY.2020,64(11):768-777.doi:10.1111/1348-0421.12842.
APA：
Li, Dongyu,Wang, Haoyong,Zhang, Pingping,Zhang, Yanhong,He, Xiang...&Wei, Congwen.(2020).Yersinia YopT inhibits RLH-mediated NF-kappa B and IRF3 signal transduction.MICROBIOLOGY and IMMUNOLOGY,64,(11)
MLA：
Li, Dongyu,et al."Yersinia YopT inhibits RLH-mediated NF-kappa B and IRF3 signal transduction".MICROBIOLOGY and IMMUNOLOGY 64..11(2020):768-777
