单位：[1]Department of Neurosurgery, Peking University International Hospital, Beijing, China,[2]Department of General Surgery, Beijing Hospital, National Center of Gerontology, Beijing, China,[3]HaploX Biotechnology, Shenzhen, China,[4]Department of Hematology, Tongji Hospital of Tongji University, Shanghai, China,[5]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China,首都医科大学附属天坛医院[6]Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, China,[7]The 2nd People’s Hospital of Tibet Autonomous Region, Lhasa, China
Background and aims:The next-generation sequencing technologies and their related assessments of circulating tumor DNA in both glioma and metastatic brain tumors remain largely limited. Methods:Based largely on a protocol approved by the institutional review board at Peking University International Hospital, the current retrospective, single-center study was conducted. Genomic DNA was extracted from blood samples or tumor tissues. With the application of NextSeq 500 instrument (Illumina), Sequencing was performed with an average coverage of 550-fold. Paired-end sequencing was employed utilized with an attempt to achieve improved sensitivity of duplicate detection and therefore to increase the detection reliability of possible fusions. Results:A total of 28 patients (21 men and 7 women) with brain tumors in the present study were involved in the study. The patients enrolled were assigned into two groups, including glioma group (n= 21) and metastatic brain tumor group (n= 7). The mean age of metastatic brain tumor group (59.86 +/- 8.85 y), (43.65 +/- 13.05 y) reported significantly higher results in comparison to that of glioma group (45.3 +/- 12.3 years) (P< 0.05). The mutant genes in metastatic brain tumor group includedALK, MDM2, ATM, BRCA1, FGFR1, MDM4andKRAS; however, there were no glioma-related mutant genes includingMGMT, IDH1, IDH2,1p/19q, and BRAF et al. Interesteringly, only two patient (28.3%) was detected blood ctDNA in metastatic brain tumor group; In contrast, blood ctDNA was found in ten glioma patients (47.6%) including 1p/19q,MDM2, ERBB2, IDH1, CDKN2A, CDK4, PDGFRA, CCNE1, MET. The characterizations ofIDHmutations in the glioma includedIDH1mutation (p.R132H) and IDH2 mutation (p.R172K). The mutation rate of IDH in tumor tissues was 37.06 +/- 8.32%, which was significantly higher than blood samples (P< 0.05). Conclusion:The present study demonstrated that the mutant genes among glioma and metastatic brain tumors are shown to be different. Moreover, the ctDNAs in the metastatic brain tumors includedALKandMDM2, and glioma-related ctDNAs included 1p/19q andMDM2followed by frequencies ofERBB2, IDH1, CDKN2A, CDK4, PDGFRA, CCNE1, MET. These ctDNAs might be biomarkers and therapeutic responders in brain tumor.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81301092, 31301118, 81900431]; National Natural Science Foundation of Tibet Autonomous Region [XZ2017ZR-ZY002]; Peking University International Hospital Research Grant [YN2017ZD02]
第一作者单位：[1]Department of Neurosurgery, Peking University International Hospital, Beijing, China,
通讯作者：
通讯机构：[6]Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, China,[7]The 2nd People’s Hospital of Tibet Autonomous Region, Lhasa, China
推荐引用方式(GB/T 7714)：
Jianfeng Liang,Wanni Zhao,Changyu Lu,et al.Next-Generation Sequencing Analysis of ctDNA for the Detection of Glioma and Metastatic Brain Tumors in Adults[J].FRONTIERS in NEUROLOGY.2020,11:doi:10.3389/fneur.2020.00544.
APA：
Jianfeng Liang,Wanni Zhao,Changyu Lu,Danni Liu,Ping Li...&Dong Yang.(2020).Next-Generation Sequencing Analysis of ctDNA for the Detection of Glioma and Metastatic Brain Tumors in Adults.FRONTIERS in NEUROLOGY,11,
MLA：
Jianfeng Liang,et al."Next-Generation Sequencing Analysis of ctDNA for the Detection of Glioma and Metastatic Brain Tumors in Adults".FRONTIERS in NEUROLOGY 11.(2020)
