单位：[1]Department of Otolaryngology, Head & Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China首都医科大学附属北京友谊医院[2]Department of General Surgery, The General Hospital of Taiyuan Iron & Steel Company, Taiyuan, China[3]Medical Department of Medical Insurance, Beijing Friendship Hospital, Capital Medical University, Beijing, China首都医科大学附属北京友谊医院[4]Imaging Department, Shanxi Medical University, Taiyuan, China[5]Department of Otolaryngology, Head & Neck Surgery, Guy’s & St Thomas NHS Hospital, London, UK[6]Department of Nursing, Peking University Shenzhen Hospital, Shenzhen, China北京大学深圳医院深圳市康宁医院深圳医学信息中心[7]Department of Otolaryngology, Head & Neck Surgery, the First Hospital of Shanxi Medical University, No.85, Jiefang South Road, Taiyuan 030001, China[8]Department of Head and Neck Surgery, Shanxi Provincial Cancer Hospital, Taiyuan, China
Three-dimensional (3D) image reconstruction of tumors based on serial histological sectioning is one of the most powerful methods for accurate high-resolution visualization of tumor structures. However, 3D histological reconstruction of whole tumor has not yet been achieved. We established a high-resolution 3D model of molecular marked whole laryngeal cancer by optimizing the currently available techniques. A series of 5,388 HE stained or immunohistochemically stained whole light microscopic images (200 x) were acquired (15.61 TB).The data set of block-face images (96.2 GB) was also captured. Direct volume rendering of serial 6.25 x light microscopy images did not demonstrate the major characteristics of the laryngeal cancer as expected. Based on fusion of two datasets, the accurate boundary of laryngeal tumor bulk was visualized in an anatomically realistic context. In the regions of interest, micro tumor structure, budding, cell proliferation and tumor lymph vessels were well represented in 3D after segmentation, which highlighted the advantages of 3D reconstruction of light microscopy images. In conclusion, generating 3D digital histopathological images of a whole solid tumor based on current technology is feasible. However, data mining strategy should be developed for complete utilization of the large amount of data generated.
基金：
Key Research and Development Program Funding of Shanxi Province (2016) [201603D121039]; Shanxi Natural Science FoundationNatural Science Foundation of Shanxi Province [201801D121344]
第一作者单位：[1]Department of Otolaryngology, Head & Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China
通讯作者：
推荐引用方式(GB/T 7714)：
JunTian,Bo Qian,Sanmei Zhang,et al.Three-dimensional reconstruction of laryngeal cancer with whole organ serial immunohistochemical sections[J].SCIENTIFIC REPORTS.2020,10(1):doi:10.1038/s41598-020-76081-7.
APA：
JunTian,Bo Qian,Sanmei Zhang,RuiGuo,Hui Zhang...&BinquanWang.(2020).Three-dimensional reconstruction of laryngeal cancer with whole organ serial immunohistochemical sections.SCIENTIFIC REPORTS,10,(1)
MLA：
JunTian,et al."Three-dimensional reconstruction of laryngeal cancer with whole organ serial immunohistochemical sections".SCIENTIFIC REPORTS 10..1(2020)
