单位：[1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室国家中心普外分中心普外五科（综合普外科）首都医科大学附属北京友谊医院[2]National Clinical Research Center of Digestive Diseases, 100050 Beijing, China首都医科大学附属北京友谊医院[3]Department of Gastrointestinal Surgery, Affiliated Hospital, Inner Mongolia Medical University, 010051 Hohhot, China
Circular RNAs (circRNAs) belong to noncoding RNAs and are widely expressed in a variety of cell species, including cancers. However, the function and mechanism of circRNAs in colorectal cancer (CRC) has not been well investigated. Here, we firstly downloaded and analyzed the circRNA expression profile of CRC from the Gene Expression Omnibus (GEO) database. And we identified 181 differentially expressed circRNAs between 10 pairs of CRC and adjacent normal tissues. Interestingly, we observed that the expression of hsa_circRNA_000166 was the top increased among these circRNAs. Then, we confirmed an upregulation of hsa_circRNA_000166 in CRC tissues and cell lines and observed that higher expression of hsa_circRNA_000166 was associated with poor 5-year survival rate of patients with CRC. Next, we investigated the function of hsa_circRNA_000166 during CRC progression by knocking down its expression. Cell growth and apoptosis assay revealed that hsa_circRNA_000166 regulated the cell growth and apoptosis in CRC cell lines. Furthermore, we identified that hsa_circRNA_000166 targeted the miR-326/LASP1 pathway using bioinformatics analysis and luciferase reporter assay. Finally, suppression of miR-326 or overexpression of LASP1 could sufficiently rescue the aberrant cell growth and apoptosis in CRC cell lines. Taken together, our results indicated that downregulation of hsa_circRNA_000166 inhibited the cell growth and facilitated apoptosis during CRC development by sponging the miR-326/LASP1 pathway.
第一作者单位：[1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[2]National Clinical Research Center of Digestive Diseases, 100050 Beijing, China[3]Department of Gastrointestinal Surgery, Affiliated Hospital, Inner Mongolia Medical University, 010051 Hohhot, China
通讯作者：
通讯机构：[1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[2]National Clinical Research Center of Digestive Diseases, 100050 Beijing, China
推荐引用方式(GB/T 7714)：
Hao Qin,Zhang Zhongtao.hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer[J].GASTROENTEROLOGY RESEARCH and PRACTICE.2020,2020:doi:10.1155/2020/8834359.
APA：
Hao, Qin&Zhang, Zhongtao.(2020).hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer.GASTROENTEROLOGY RESEARCH and PRACTICE,2020,
MLA：
Hao, Qin,et al."hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer".GASTROENTEROLOGY RESEARCH and PRACTICE 2020.(2020)
