单位：[1]Key Laboratory of Semiconductor Material Sciences, Beijing Key Laboratory of Low Dimensional Semiconductor Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, People’s Republic of China[2]College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China[3]Department of Cardiology, Peking University People’s Hospital, Beijing 100044, People’s Republic of China[4]China-Japan Friendship Hospital, Beijing 100029, People’s Republic of China
The high electron mobility transistor (HEMT)-based biosensors are highly competitive in the ultimate application of portable and point-of-care testing. Herein, we have demonstrated highly sensitive and real-time detection of cardiac troponin I (cTnI), a biomarker for the diagnosis of acute myocardial infarction (AMI) using AlGaAs/GaAs HEMT-based biosensors. The device has achieved a lower detection limit of 1 pg/ml in the buffer solution and less than 30 s response time, which demonstrated significant promise in the early diagnosis and screening of AMI. In addition, our results are consistent with the enzyme-linked immunosorbent assay according to the AMI patient's blood test results. Furthermore, by comparing the two HEMT structures, we also calculated the equilibrium dissociation constant (K-D) of the cTnI and cTnI antibody and analyzed the sensing mechanism. The results show that this method is very promising for early diagnosis of AMI.
基金：
National Key Research and Development Program of China [2017YFB0405400]; Capital Health Research and Development of Special [2020-2-4084]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61874105]; Youth Innovation Promotion Association CAS [Y201925]
第一作者单位：[1]Key Laboratory of Semiconductor Material Sciences, Beijing Key Laboratory of Low Dimensional Semiconductor Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, People’s Republic of China[2]College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory of Semiconductor Material Sciences, Beijing Key Laboratory of Low Dimensional Semiconductor Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, People’s Republic of China[2]College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式(GB/T 7714)：
Luo Jiaming,Li Sufang,Xu Mengke,et al.Real-time detection of cardiac troponin I and mechanism analysis of AlGaAs/GaAs high electron mobility transistor biosensor[J].AIP ADVANCES.2020,10(11):doi:10.1063/5.0027553.
APA：
Luo, Jiaming,Li, Sufang,Xu, Mengke,Guan, Min,Yang, Mengxi...&Zeng, Yiping.(2020).Real-time detection of cardiac troponin I and mechanism analysis of AlGaAs/GaAs high electron mobility transistor biosensor.AIP ADVANCES,10,(11)
MLA：
Luo, Jiaming,et al."Real-time detection of cardiac troponin I and mechanism analysis of AlGaAs/GaAs high electron mobility transistor biosensor".AIP ADVANCES 10..11(2020)
