单位：[1]Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China[2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China[3]Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, China
CD40 ligand (CD40L) and B-cell activating factor (BAFF) play important roles in the function of B cells. However, the difference of their regulatory effects remains obscure. In this study, we used anti-CD40 to imitate CD40L and investigated the different regulatory effects of CD40L and BAFF on the function of B cells. In the functional analyses, both anti-CD40 and BAFF significantly enhanced the survival and differentiation of B cells, and slightly increased the activation and proliferation. However, in the transcriptome analysis, anti-CD40 and BAFF exerted very different regulation on the gene expression profile of B cells. Anti-CD40 upregulated the expression of genes related to the adaptive immune function of B cells, but BAFF enhanced the genes associated with the innate immune function. Furthermore, the effect analysis of the combination of anti-CD40 or BAFF with anti-IgM also demonstrated that anti-CD40 could cooperate with anti-IgM to promote the proliferation of B cells, but BAFF could not do it. The mechanism study revealed that the different effects of anti-CD40 and BAFF on B cells were resulting from the different modulation on NF-kappa B, ERK1/2, and PI3K-AKT signaling pathways. Collectively, the results suggest that CD40L mainly promotes adaptive immune function of B cells, but BAFF primarily enhances innate immune function.
基金：
National Natural Science Foundation of China, ChinaNational Natural Science Foundation of China (NSFC) [81603361]
第一作者单位：[1]Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
通讯作者：
通讯机构：[1]Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China[2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China[*1]Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 11 Beisanhuan East Road, Chaoyang District, Beijing 100029, China (Z. Zhu)[*2]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China
推荐引用方式(GB/T 7714)：
Guo Ran,Wang Wenxuan,Yu Lanzhi,et al.Different regulatory effects of CD40 ligand and B-cell activating factor on the function of B cells[J].INTERNATIONAL IMMUNOPHARMACOLOGY.2021,91:doi:10.1016/j.intimp.2020.107337.
APA：
Guo, Ran,Wang, Wenxuan,Yu, Lanzhi,Zhu, Zhixiang&Tu, Pengfei.(2021).Different regulatory effects of CD40 ligand and B-cell activating factor on the function of B cells.INTERNATIONAL IMMUNOPHARMACOLOGY,91,
MLA：
Guo, Ran,et al."Different regulatory effects of CD40 ligand and B-cell activating factor on the function of B cells".INTERNATIONAL IMMUNOPHARMACOLOGY 91.(2021)
