单位：[1]Toulouse Purpan Medical School, Toulouse, France[2]Department of Neurology, Massachusetts General Hospital, Boston, MA, USA[3]Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室神经内科神经内科首都医科大学附属北京友谊医院[4]Harvard Medical School, Boston, MA, USA
Objective To identify circular RNAs as candidates for differential expression in the middle temporal (MT) cortex in a well-characterized cohort with contrasting Alzheimer disease (AD) pathology and cognition. Top screen candidates were assessed for proof of circularity and then quantified by qPCR in a larger number of samples. Methods An initial RNA sequencing screen was performed on n = 20 frozen human tissue samples. Filters were applied to select candidate circular RNAs for further investigation. Frozen human tissue samples were selected for global AD pathology burden and global cognition scores (n = 100). Linear and divergent primers were used to assess circularity using RNaseR digestion. RT-qPCR was performed to quantify relative hsa_circ_0131235 abundance. Results Eleven circular RNAs were selected for further investigation. Four candidates produced circular RNA primers with appropriate efficiencies for qPCR. RNaseR treatment and analysis by both basic PCR and qPCR confirmed hsa_circ_0131235 circularity. There was a significant main effect of AD pathology on hsa_circ_0131235 expression. Conclusions Elevated hsa_circ_0131235 expression in the MT cortex was significantly associated with AD pathology.
基金：
National Institute on AgingUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Aging (NIA)
第一作者单位：[1]Toulouse Purpan Medical School, Toulouse, France
通讯作者：
通讯机构：[2]Department of Neurology, Massachusetts General Hospital, Boston, MA, USA[4]Harvard Medical School, Boston, MA, USA[*1]Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.
推荐引用方式(GB/T 7714)：
Bigarré I.M,Trombetta B.A,Guo Y.-J,et al.IGF2R circular RNA hsa_circ_0131235 expression in the middle temporal cortex is associated with AD pathology[J].BRAIN and BEHAVIOR.2021,11(4):doi:10.1002/brb3.2048.
APA：
Bigarré, I.M,Trombetta, B.A,Guo, Y.-J,Arnold, S.E&Carlyle, B.C.(2021).IGF2R circular RNA hsa_circ_0131235 expression in the middle temporal cortex is associated with AD pathology.BRAIN and BEHAVIOR,11,(4)
MLA：
Bigarré, I.M,et al."IGF2R circular RNA hsa_circ_0131235 expression in the middle temporal cortex is associated with AD pathology".BRAIN and BEHAVIOR 11..4(2021)
