单位：[1]Department of Oncology, The First People’s Hospital of Qujing/The Qujing Affiliated Hospital of Kunming Medical University, Qujing, Yunnan, China[2]Department of Thoracic Surgery, The First People’s Hospital of Qujing/The Qujing Affiliated Hospital of Kunming Medical University, Qujing, Yunnan, China[3]Department of Radiation Oncology, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China[4]Institute of Respiratory Medicine, Chinese Academy of Medicine Sciences, Beijing, China[5]National Center for Respiratory Disease, Beijing, China
BACKGROUND: Radiotherapy is one of main useful therapies in non-small cell lung cancer (NSCLC). Nevertheless, the underlying mechanism between NSCLC cell radiosensitivity and effective treatment remains unclear. OBJECTIVE: The aim is to explore the relationship between circular (circ) RNA and NSCLC cell radiosensitivity. METHODS: CircRNA plasmacytoma variant translocation 1 (PVT1) and microRNA (miR)-1208 expression in NSCLC cells were assessed using quantitative reverse transcriptase PCR (qRT-PCR). NSCLC cells were transfected with si-PVT1 or miR-1208 inhibitor and then exposed to irradiation. Cellular biology behaviors were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL), colony formation, invasion and western blot. Additionally, binding between circPVT1 and miR-1208 was testified by dual-luciferase reporter and RIP assay. RESULTS: CircPVT1 was upregulated in NSCLC cells after irradiation treatment. Silencing circPVT1 induced inhibition of NSCLC cell growth and invasion, accompanied by cell apoptosis and gamma-H2AX expression. Moreover, NSCLC cell proliferation and invasion was further inhibited by irradiation treatment in circPVT1-silenced cells, indicating a strong radiosensitivity of NSCLC cells. CircPVT1 functions as a competing endogenous RNA of miR-1208. Silencing miR-1208 reversed NSCLC cell sensitivity response to irradiation and activated PI3K/AKT/mTOR pathway in circPVT1-silenced cells. CONCLUSIONS: Silencing circPVT1 enhanced radiosensitivity of NSCLC cells by sponging miR-1208.
基金：
Mechanism of Changes in Immune Status of High-incidence Lung Cancer in the Coal-burning Area of Eastern Yunnan on Comprehensive Treatment, Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special General Project [2019FE001 (-106)]; SPP1 Regulates Female Lung Cancer Cell Stem Resistance and its Mechanism of Drug Resistance in Xuanwei Area [2019J1313]; Yunnan Provincial Department of Science and Technology-Kunming Medical University Applied Basic Research Joint Special Fund General Project; Ministry of Science and Technology: A Multi-Center Open Clinical Study of Stereotactic Body Radiotherapy Combined with Immune Checkpoint Inhibitors in the Treatment of Early Non-Small Cell Lung Cancer [2018YFC1313202]
第一作者单位：[1]Department of Oncology, The First People’s Hospital of Qujing/The Qujing Affiliated Hospital of Kunming Medical University, Qujing, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Thoracic Surgery, The First People’s Hospital of Qujing/The Qujing Affiliated Hospital of Kunming Medical University, Qujing, Yunnan, China[3]Department of Radiation Oncology, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China[4]Institute of Respiratory Medicine, Chinese Academy of Medicine Sciences, Beijing, China[5]National Center for Respiratory Disease, Beijing, China[*1]Department of Thoracic Surgery, The First People’s Hospital of Qujing/The Qujing Affiliated Hospital of Kunming Medical University, No.1 Yuanlin Road, Qilin District, Qujing 655000, Yunnan, China.[*2]Department of Radiation Oncology, Center of Respiratory Medicine, China-Japan Friendship Hospital.Institute of Respiratory Medicine, Chinese Academy of Medicine Sciences.National Center for Respiratory Disease, No.2 Yinghuadong Street, Chaoyang, Beijing 100029, China.
推荐引用方式(GB/T 7714)：
Huang Meifang,Li Tianqian,Wang Qing,et al.Silencing circPVT1 enhances radiosensitivity in non-small cell lung cancer by sponging microRNA-1208[J].CANCER BIOMARKERS.2021,31(3):263-279.doi:10.3233/CBM-203252.
APA：
Huang Meifang,Li Tianqian,Wang Qing,Li Chongxin,Zhou Huahua...&Zhu Guangying.(2021).Silencing circPVT1 enhances radiosensitivity in non-small cell lung cancer by sponging microRNA-1208.CANCER BIOMARKERS,31,(3)
MLA：
Huang Meifang,et al."Silencing circPVT1 enhances radiosensitivity in non-small cell lung cancer by sponging microRNA-1208".CANCER BIOMARKERS 31..3(2021):263-279
