OBJECTIVE: To investigate the efficacy and mechanism of the Qingre Huayu Fang on atherosclerotic vulnerable plaque in apolipoprotein E (ApoE) knockout mice through the ubiquitin proteasome pathway. METHODS: Sixty 8-week-old C57BL/6J ApoE knockout mice were fed a high-fat for 12 weeks and randomly divided into four treatment groups (n = 15 each): high-fat control, bortezomib (a proteasome inhibitor), bortezomib combined with Qingre Hua-yu Fang, and Qingre Huayu Fang alone. Aortic sections were examined for plaque development, inflammatory cell infiltration, type I / III collagen expression and immunohistochemical staining of CD40L, nuclear factor-kappa B (NF-kappa B)/P65 and ubiquitin. RESULTS: Mice in the high-fat control group had obvious atherosclerosis, with increased aortic plaque area. The degree of atherosclerosis of the atherosclerotic plaque was reduced in all of the treatment groups that received bortezomib and/or Duzhong (Cortex Eucommiae) Qingre Huayu. The expression of NF-.B, CD40L and ubiquitin were all reduced in the group that received combination bortezomib + Qingre Huayu Fang. CONCLUSION: The Qingre Huayu Fang inhibited aortic atherosclerosis in mice through a mechanism that may involve inhibition of the ubiquitin proteasome pathway. (C) 2021 JTCM. All rights reserved.
基金：
Guizhou Basic Research Project (Study on the Effect of Eucommia ulmoides Extract through Nuclear Factor NF-KB Molecular Pathway to Interfere with Atherosclerosis, Qian Heke Foundation) [1190]; National Natural Science Foundation Regional Fund Project [81173420, 81760071]
第一作者单位：[1]Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang 520000, China
通讯作者：
通讯机构：[2]Department of Hypertension，Beijing An-zhen Hospital, Beijing 100029,China[*1]Department of Hypertension，Beijing Anzhen Hospital，Beijing 100029，China.
推荐引用方式(GB/T 7714)：
PANG Jun,CHENG Wenli,PENG Jingbing,et al.Efficacy of Qingre Huayu Fang on atherosclerotic vulnerable plaque in apolipoprotein E knockout mice: proteasome pathway involvement[J].JOURNAL of TRADITIONAL CHINESE MEDICINE.2021,41(3):432-437.doi:10.19852/j.cnki.jtcm.2021.03.011.
APA：
PANG Jun,CHENG Wenli,PENG Jingbing,LI Hong,WU Qiang...&HUANG Jing.(2021).Efficacy of Qingre Huayu Fang on atherosclerotic vulnerable plaque in apolipoprotein E knockout mice: proteasome pathway involvement.JOURNAL of TRADITIONAL CHINESE MEDICINE,41,(3)
MLA：
PANG Jun,et al."Efficacy of Qingre Huayu Fang on atherosclerotic vulnerable plaque in apolipoprotein E knockout mice: proteasome pathway involvement".JOURNAL of TRADITIONAL CHINESE MEDICINE 41..3(2021):432-437
