Purpose: This study aimed to assess stapes visualization using an ultra-high resolution computed tomography (UHRCT). Method: Sixty ears from 30 cadaveric human heads were scanned by both U-HRCT and 128-section multislice CT (MSCT) with clinical parameters. Image quality of the stapes head, anterior and posterior crura, footplate, incudostapedial joint and stapedial muscle within the pyramidal eminence was scored using a 3-point Likert scale. Linear measurements of the stapes configuration were performed on U-HRCT. Results: The interobserver agreement for image qualitative score on U-HRCT was good to excellent (interobserver agreement coefficients 0.65-0.86). With the exception of the stapes head, U-HRCT achieved significantly higher qualitative scores than MSCT across all anatomical structures (Ps < 0.05). The total height of the stapes was measured to be 3.48 +/- 0.33 mm. The height and width of the obturator foramen were 1.77 +/- 0.28 mm and 2.19 +/- 0.33 mm, respectively. The widths of the anterior and posterior crura were 0.20 +/- 0.06 mm and 0.22 +/- 0.06 mm, respectively. The thickness of the footplate was 0.22 +/- 0.06 mm, and the angle of the incudostapedial joint was 95.91 +/- 10.69 degrees. Conclusions: U-HRCT is capable of delineating fine structures of the stapes and provides linear data on dimensions of the stapes, which could be helpful for detecting stapes disease and making individualized surgical plans in the clinical setting.