单位：[1]Department of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China,[2]School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China,[3]Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China,[4]Department of Cardiology, China-Japan Friendship Hospital, Beijing, China,[5]Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China,[6]Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China,[7]Department of Nursing, Army Medical Center of PLA, Chongqing, China
Developing a three-dimensional (3D) visualization of the kidney at the whole-mount scale is challenging. In the present study, we optimized mouse whole-mount kidney clearing, which improved the transparency ratio to over 90% based on organ-specific perfusion (OSP)-clear, unobstructed brain imaging cocktails and computational analysis (CUBIC). The optimized OSP-CUBIC-compatible 3D immunostaining and imaging simultaneously visualized the high-resolution 3D structure of the whole-mount renal microvascular, glomerulus, and accompanying wrapped traveling sympathetic nerves in mice. A mouse model of pressure overload-induced heart failure (HF) was then established by minimally invasive transverse aortic constriction (MTAC). Further 3D quantification revealed renal sympathetic hyperinnervation (6.80 +/- 1.04% vs. 3.73 +/- 0.60%, P < 0.05) in mice with HF. In conclusion, this newly developed whole-organ tissue clearing and imaging system provides comprehensive information at the whole-mount scale and has great potential for kidney research. Our data suggest that renal sympathetic hyperinnervation is involved in HF associated with renal dysfunction.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91639110]; National Key Clinical Specialty Discipline Construction Program [2020-QTL-009]; Natural Science Foundation of Beijing MunicipalityBeijing Natural Science Foundation [7172195]
第一作者单位：[1]Department of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China,
通讯作者：
通讯机构：[1]Department of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China,[4]Department of Cardiology, China-Japan Friendship Hospital, Beijing, China,[5]Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China,[6]Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China,
推荐引用方式(GB/T 7714)：
Chao Wu,Fang Yan,Min Li,et al.Whole-Mount Kidney Clearing and Visualization Reveal Renal Sympathetic Hyperinnervation in Heart Failure Mice[J].FRONTIERS in PHYSIOLOGY.2021,12:doi:10.3389/fphys.2021.696286.
APA：
Chao Wu,Fang Yan,Min Li,Yimin Tu,Ziyu Guo...&Jingang Zheng.(2021).Whole-Mount Kidney Clearing and Visualization Reveal Renal Sympathetic Hyperinnervation in Heart Failure Mice.FRONTIERS in PHYSIOLOGY,12,
MLA：
Chao Wu,et al."Whole-Mount Kidney Clearing and Visualization Reveal Renal Sympathetic Hyperinnervation in Heart Failure Mice".FRONTIERS in PHYSIOLOGY 12.(2021)
