单位：[1]Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China[2]Department of Pathology, China-Japan Friendship Hospital, Beijing, China[3]Department of Information Management, China-Japan Friendship Hospital, Beijing, China[4]Department of Thoracic Oncology, National Cancer Center Hospital, Tokyo, Japan
Background: Early-stage female lung adenocarcinoma is the most common type of lung cancer encountered in thoracic surgery departments. Tumor-node-metastasis (TNM) staging does not adequately explain a significant stratification phenomenon in the prognosis of patients with stage I lung adenocarcinoma. We aimed to investigate the contributory role of miR-940 in the prognosis prediction. Methods: We analyzed the microRNA (miRNA) expression level in tumor tissues (high-risk group vs. low-risk group) from 12 non-smoking female patients with stage I lung adenocarcinoma using miRNA array. Bioinformatic analyses of miR-940 were also carried out based on the public database. Then, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) tests of the tissue samples were further validated. And miR-940's function was analyzed and potential target genes were predicted. Results: In all, 24 miRNAs were found to be significantly different between the high-risk group and low-risk group. The expression level of miR-940 was lower in tumor tissue (P=0.011), and the survival rate in the high miR-940 group was higher [hazard ratio (HR) =0.688; P=0.011]. Gene Ontology (GO) analysis showed that the assembly functions of targets regulated by miR-940 were mainly enriched in regulation of myeloid cell differentiation, G1/S transition of mitotic cell cycle, and cellular response to environmental stimulus. miR-940 is involved in transforming growth factor-beta (TGF-beta) signaling pathway; TNF signaling pathway; and estrogen signaling pathway. The number of lung adenocarcinoma cells (A549) was significantly decreased after miR-940 was transfected. Ten epithelial-to-mesenchymal-transition (EMT)-associated genes (MMP9, ZEB1, CDH1, KRT8, KRT18 KET19, TWIST1, VIM, SNAI1, and SNAI2) were found to be significantly related to miR-940. Conclusions: The present study showed that miR-940 might be a protective factor for positive prognosis in early stage nonsmoking female lung adenocarcinoma, with transforming growth factor-beta (TGF-beta) pathway, TNF pathway, and matrix metalloprotein (MMP9) being potential targets.
基金：
China-Japan Friendship Hospital [2015-1-QN-20]; Overseas Scholarship [2014-QTL-032]; Peking science and technology fund [Z191100006619008]; Elite Medical Professionals project of China-Japan Friendship Hospital [ZRJY2021-GG02]
第一作者单位：[1]Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China[3]Department of Information Management, China-Japan Friendship Hospital, Beijing, China[*1]Department of Thoracic Surgery, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang, Beijing 100029, China.[*2]Chaozeng Si. Department of Information Management, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang, Beijing 100029, China.[*3]Department of Thoracic Surgery, China-Japan Friendship Hospital, 2 Yinghua East Road, Chaoyang, Beijing 100029, China.
推荐引用方式(GB/T 7714)：
Ma Qianli,Zhang Jin,Huang Jingjing,et al.Decreased miR-940 expression can predict a negative prognosis in early-stage nonsmoking female lung adenocarcinoma[J].TRANSLATIONAL LUNG CANCER RESEARCH.2021,10(11):4293-+.doi:10.21037/tlcr-21-906.
APA：
Ma, Qianli,Zhang, Jin,Huang, Jingjing,Wang, Xiaowei,Xiao, Fei...&Liang, Chaoyang.(2021).Decreased miR-940 expression can predict a negative prognosis in early-stage nonsmoking female lung adenocarcinoma.TRANSLATIONAL LUNG CANCER RESEARCH,10,(11)
MLA：
Ma, Qianli,et al."Decreased miR-940 expression can predict a negative prognosis in early-stage nonsmoking female lung adenocarcinoma".TRANSLATIONAL LUNG CANCER RESEARCH 10..11(2021):4293-+
