单位：[1]Graduate School of Beijing University of Chinese Medicine, Beijing, China[2]Andrology Department, China-Japan Friendship Hospital, Beijing, China[3]Department of Andrology, Shunyi Hospital of Beijing Traditional Chinese medicine hospital, Beijing, China[4]Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China
Zuogui Wan (ZGW) is a common prescription medication used in traditional Chinese medicine (TCM) to significantly improve the sperm quality and treat male infertility. This study evaluated the repair effect of ZGW and Levocarnitine (LEV) on GC1-spg cell injury induced by Glucosides of Tripterygium WilforDII Hook (GTW). The results showed that the ultrastructure and apoptosis rate of GC1- spg cells in LEV and ZGW group were considerably better than GTW. The transcriptional and translational level of CYP1A1, CYP17A1, androgen receptor (AR), SRD5A2 and proliferating cell nuclear antigen (PCNA) in GC-1spg cells of the LEV group were considerably elevated than GTW group (p < 0.05 or 0.01). Furthermore, the transcriptional and translational levels of CYP19A1, CYP17A1, AR, SRD5A2 and PCNA in GC-1spg cells in ZGW group were found to be considerably elevated than the LEV group (p < 0.05 or 0.01). The findings indicate that ZGW and LEV could increase the expression of PCNA, CYP17A1, CYP19A1, SRD5A2 and AR at transcriptional and translational levels, inhibit GC-1spg cell apoptosis and promoting cell proliferation, and the effect of ZGW was found to be significantly better than that of LEV.
基金：
National Natural Science Foundation of China [81273756, 81774320]
第一作者单位：[1]Graduate School of Beijing University of Chinese Medicine, Beijing, China[2]Andrology Department, China-Japan Friendship Hospital, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[4]Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China[*1]Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
推荐引用方式(GB/T 7714)：
Bao Binghao,Yang Zhen,Deng Sheng,et al.Zuogui Wan improves spermatogenesis of GC1-spg cells through modulating AR-related pathways[J].ANDROLOGIA.2022,54(6):doi:10.1111/and.14407.
APA：
Bao, Binghao,Yang, Zhen,Deng, Sheng,Dai, Hengheng,Feng, Junlong...&Wang, Jisheng.(2022).Zuogui Wan improves spermatogenesis of GC1-spg cells through modulating AR-related pathways.ANDROLOGIA,54,(6)
MLA：
Bao, Binghao,et al."Zuogui Wan improves spermatogenesis of GC1-spg cells through modulating AR-related pathways".ANDROLOGIA 54..6(2022)
