单位：[1]The Heart Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China 100050临床科室心血管中心心外科首都医科大学附属北京友谊医院[2]Cardiovascular Surgery Ⅱ, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China 100045首都医科大学附属北京儿童医院
The objective was to identify the differential expressed miRNA during cerebral ischemia-reperfusion injury (CIRI) process, thereby assisting in elucidating the mechanism of CIRI development and providing a potential target for CIRI prevention and treatment. Six mice were randomly assigned to two groups: control group and CIRI model group. A global cerebral IR model by four-vessel occlusion was prepared among the CIRI model group. Brain tissues were collected 48 h after reperfusion. Total RNA was extracted for each sample. miRNA inicroarrays were employed to detect the differentially expressed miRNA between the CIRI group and the control group. One differentially expressed miRNA was selected for verification by PCR. Compared with the control group, 69 miRNAs were significantly differential expressed in samples of the CIRI group, among which 50 miRNAs were upregulated and 19 miRNAs were downregulated. The real-time qPCR results indicated that the results of the miRNA microarray were reliable. A number of miRNAs were significantly regulated in the CIRI model, which suggested that miRNA was closely associated with the pathological alterations after ischemia. These identified miRNAs may provide directions and targets for the future pathological research of CIRI.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81371443, 8140030]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7122056, 7142049, 7142137, 7152045, 7182042]; Xinjiang Uygur Autonomous Region Natural Science Foundation [2014211A063]; Fund of Capital Medical University Clinical-Basic Cooperation Research [13JL26]; Beijing Outstanding Talent Training Project [2014000021469G233]; Beijing Health Bureau High-level Talent Training Project [2014-3-043]; Beijing Municipal Administration of Hospitals Incubating Program [PX2016046]; Special Fund of The Pediatric Medical Coordinated Development Center of Beijing Municipal Administration [XTYB201819]; Research on the application of clinical characteristics of the Beijing Municipal Science and Technology Commission [Z171100001017048]; Beijing Municipal Education Commission Science and Technology Plan General Project [KM201910025010]
第一作者单位：[1]The Heart Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China 100050
通讯作者：
通讯机构：[2]Cardiovascular Surgery Ⅱ, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China 100045[*1]Cardiovascular SurgerⅡ,Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China 100045
推荐引用方式(GB/T 7714)：
Yaping Zhang,Nan Ding,Hanlu Yi,et al.Identification of differentially expressed miRNA 48 h after cerebral ischemia-reperfusion injury in mice by the technique of miRNA microarray[J].CANADIAN JOURNAL of PHYSIOLOGY and PHARMACOLOGY.2020,98(12):855-860.doi:10.1139/cjpp-2019-0701.
APA：
Yaping Zhang,Nan Ding,Hanlu Yi,Yudong Zhao,Zankai Ye...&Yaobin Zhu.(2020).Identification of differentially expressed miRNA 48 h after cerebral ischemia-reperfusion injury in mice by the technique of miRNA microarray.CANADIAN JOURNAL of PHYSIOLOGY and PHARMACOLOGY,98,(12)
MLA：
Yaping Zhang,et al."Identification of differentially expressed miRNA 48 h after cerebral ischemia-reperfusion injury in mice by the technique of miRNA microarray".CANADIAN JOURNAL of PHYSIOLOGY and PHARMACOLOGY 98..12(2020):855-860
