单位：[1]Department of Ophthalmology, University of California, San Francisco, San Francisco, California, United States[2]Department of Ophthalmology, Zuckerberg San Francisco General Hospital and Trauma Center, San Francisco, California, United States[3]Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, School of Optometry, Shenzhen University, Shenzhen, China[4]Department of Ophthalmology, Charoenkrung Pracharak Hospital, Bangkok, Thailand[5]Department of Ophthalmology, China-Japan Friendship Hospital, Beijing, China[6]State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China[7]Department of Ophthalmology, Affiliated Hospital of Guizhou Medical University, Guiyang, China
PURPOSE. To investigate the relationship between proangiogenic and inflammatory cytokines in concurrent vitreous, aqueous, and plasma samples from patients with proliferative diabetic retinopathy (PDR). METHODS. Vitreous, aqueous, and plasma samples were analyzed using multiplex immunoassay for 10 PDR-related cytokines (IL-6, IL-8, TNF-alpha, monocyte chemoattractant protein-1 [MCP-1], macrophage inflammatory protein-1 beta [MIP-1 beta], VEGF receptor 1 [Flt-1], placental growth factor [PlGF], VEGF-A, VEGF-C, VEGF-D). A total of 17 patients with PDR and 7 controls were included. The primary outcome was correlation of cytokines in vitreous, aqueous, and plasma. The secondary outcome was the comparison of cytokine levels in controls and diabetics with and without recent anti-VEGF injection. RESULTS. The following factors were elevated in diabetics compared with controls: vitreous IL-6, IL-8, TNF-alpha, MCP-1, MIP-1 beta, PlGF, and VEGF-A; and aqueous IL-6, IL-8, PlGF, and VEGF-C (all P < 0.05). Vitreous and aqueous IL-8, PlGF, and VEGF-A were significantly correlated in patients with PDR (all P < 0.05). Plasma cytokines were not correlated with those in vitreous and aqueous (all P > 0.05). Vitreous and aqueous IL-6, IL-8, TNF-alpha, PlGF, and VEGF-A differed among controls and diabetics with and without recent anti-VEGF injection (all P < 0.05). In one-to-one comparisons, aqueous VEGF-A levels were lower in diabetic patients who had recent anti-VEGF injection compared with those who did not (P = 0.01). CONCLUSIONS. In this proof-of-concept study, IL-8, VEGF-A, and PlGF demonstrated a strong correlation in vitreous and aqueous of patients with PDR. The aqueous may serve as a proxy for vitreous for some cytokines involved in PDR. Recent anti-VEGF injections decreased VEGF-A levels in aqueous, but did not significantly affect other cytokines, suggesting a role for other targeted therapies in PDR management.
基金：
National Eye Institute, Core Grant for Vision Research [EY002162, 1R01EY024004]; Research to Prevent Blindness, Inc. (New York, NY, USA)Research to Prevent Blindness (RPB); That Man May See, Inc. (San Francisco, CA, USA)
第一作者单位：[1]Department of Ophthalmology, University of California, San Francisco, San Francisco, California, United States[2]Department of Ophthalmology, Zuckerberg San Francisco General Hospital and Trauma Center, San Francisco, California, United States
通讯作者：
通讯机构：[1]Department of Ophthalmology, University of California, San Francisco, San Francisco, California, United States[2]Department of Ophthalmology, Zuckerberg San Francisco General Hospital and Trauma Center, San Francisco, California, United States[*1]Department of Ophthalmology, University of California, San Francisco, 10 Koret Way, K301, San Francisco, CA 94143-0730, USA
推荐引用方式(GB/T 7714)：
Wu Frances,Phone Audrey,Lamy Ricardo,et al.Correlation of Aqueous, Vitreous, and Plasma Cytokine Levels in Patients With Proliferative Diabetic Retinopathy[J].INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.2020,61(2):doi:10.1167/iovs.61.2.26.
APA：
Wu, Frances,Phone, Audrey,Lamy, Ricardo,Ma, Dahui,Laotaweerungsawat, Sawarin...&Stewart, Jay M..(2020).Correlation of Aqueous, Vitreous, and Plasma Cytokine Levels in Patients With Proliferative Diabetic Retinopathy.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,61,(2)
MLA：
Wu, Frances,et al."Correlation of Aqueous, Vitreous, and Plasma Cytokine Levels in Patients With Proliferative Diabetic Retinopathy".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 61..2(2020)
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