单位：[a]Department of Otolaryngology Head Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing首都医科大学附属北京友谊医院[b]Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Chengdu Medical College[c]School of Biomedical Sciences, Chengdu Medical College, Chengdu, China
Our previous studies have confirmed that alpha 2 delta 1 has the potential to function as a cancer stem cell marker, and CACNA2D1 is the coding gene of alpha 2 delta 1. But it is unclear how microRNAs regulate the expression of the CACNA2D1 gene in laryngeal cancer cells. We detected the expressions of alpha 2 delta 1 protein, microRNA-107, and CACNA2D1 in 40 pairs of laryngeal cancer tissues and adjacent normal tissues. Laryngeal squamous cell carcinoma cells, TU212 and TU686, were cultured and transfected in the blank control group, the agomiR negative control group, the agomiR-107 group, the antagomiR negative control group, or the antagomiR-107 group, and the dual-luciferase reporter assay was employed to assess the regulatory effect of microRNA-107 on CACNA2D1. Then, the effects of microRNA-107 on the biological function of laryngeal squamous cell carcinoma cells were detected by qRT-PCR, Western blot, MTT, cell migration/invasion assay, and cell colony-formation assay. Our data suggested that the protein level of alpha 2 delta 1, encoded by CACNA2D1, in laryngeal carcinoma tissues was higher than that in adjacent normal tissues, while the expression of microRNA-107 was significantly decreased in laryngeal carcinoma tissues. The dual-luciferase reporter gene assay confirmed that microRNA-107 bound to the 3 '-UTR two positions (202-209, 902-908) of CACNA2D1 mRNA. Moreover, the expression of CACNA2D1 and alpha 2 delta 1 protein were significantly decreased in TU212 and TU686 cells transfected with microRNA-107 expression vectors (P < 0.05), and proliferation, clone formation, migration, and invasion of these cells were also reduced. Furthermore, after knocking down microRNA-107, exactly opposite results were obtained. Overexpression of microRNA-107 can inhibit the proliferation and invasion of laryngeal carcinoma cells in vitro.
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding [XLMX201701]; Research Fund of Chengdu Medical College [CYZ17-33]
第一作者单位：[a]Department of Otolaryngology Head Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing[b]Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Chengdu Medical College
通讯作者：
推荐引用方式(GB/T 7714)：
Huang Chaoping,Wang Zhenxiao,Zhang Kun,et al.MicroRNA-107 inhibits proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1 in vitro[J].ANTI-CANCER DRUGS.2020,31(3):260-271.doi:10.1097/CAD.0000000000000865.
APA：
Huang, Chaoping,Wang, Zhenxiao,Zhang, Kun,Dong, Yanbo,Zhang, Aobo...&Liu, Liangfa.(2020).MicroRNA-107 inhibits proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1 in vitro.ANTI-CANCER DRUGS,31,(3)
MLA：
Huang, Chaoping,et al."MicroRNA-107 inhibits proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1 in vitro".ANTI-CANCER DRUGS 31..3(2020):260-271
