Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head
单位：[1]Department of Orthopaedic Surgery, The Second Affiliated Hospital andYuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China[2]Graduate School of Peking Union Medical College, Beijing, China[3]Department of Orthopaedic Surgery, China-Japan Friendship Hospital,Beijing, China[4]Beijing University of Chinese Medicine, Beijing, China[5]PekingUniversity China-Japan Friendship Institute of Clinical Medicine, Beijing,China
Background Glucocorticoid-induced osteonecrosis of the femoral head (ONFH) is closely associated with the dysfunction of the bone microvascular endothelial cells (BMECs). The present study investigated the angiogenic and apoptotic activity of the BMECs in glucocorticoid-induced ONFH. Methods This study enrolled a total of 12 patients, six of whom were assigned to the ONFH group whereas the other six served as the control group. The ONFH group was composed of patients with glucocorticoid-induced ONFH while the control group had femoral neck fractures. BMECs were isolated from the subchondral region of the femoral head. Cell proliferation, cell viability, tube formation assay, Transwell assay, TUNEL assay, and Western blot analysis were performed. Results BMECs of the two groups were successfully isolated and identified. No significant differences were noticed in BMECs proliferation between the two groups. However, compared to the control, cell viability, tube formation, and migration of BMECs were significantly decreased and the number of TUNEL positive cells was markedly increased in the ONFH group. In the ONFH group, it was also noted that the amount of Bax and cleaved-caspase3 was elevated while that of Bcl-2 was reduced. Conclusion The findings of our study revealed that BMECs obtained from the glucocorticoid-induced ONFH patients had decreased angiogenic and increased apoptotic activities, which could explain the pathogenesis and progression of glucocorticoid-induced ONFH.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81703896, 81673776]; Beijing municipal science and technology commissionBeijing Municipal Science & Technology Commission [Z171100001017209]; Capital Health Research and Development of Special [2016-2-4062]; Wenzhou Municipal Science and Technolgoy Burea [Y20180030]; Zhejiang Medical and Health Science and Technology Project [2020KY181]
第一作者单位：[1]Department of Orthopaedic Surgery, The Second Affiliated Hospital andYuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China[2]Graduate School of Peking Union Medical College, Beijing, China
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推荐引用方式(GB/T 7714)：
Huachen Yu,Pei Liu,Wei Zuo,et al.Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head[J].BMC MUSCULOSKELETAL DISORDERS.2020,21(1):doi:10.1186/s12891-020-03225-1.
APA：
Huachen Yu,Pei Liu,Wei Zuo,Xiaowei Sun,Hongzhi Liu...&Qidong Zhang.(2020).Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head.BMC MUSCULOSKELETAL DISORDERS,21,(1)
MLA：
Huachen Yu,et al."Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head".BMC MUSCULOSKELETAL DISORDERS 21..1(2020)
