Purpose: To characterize the aqueous levels of inflammation and ischemia-related biomarkers in a spectrum of retinal ischemic conditions, including neovascular glaucoma (NVG) with stable iris neovascularization after pan retinal photocoagulation (PRP) and anti-VEGF treatment. Methods: Aqueous samples were collected from 139 eyes including NVG (n = 12), stable NVG (n = 26), CRVO (n = 11), NPDR (n = 18), PACG (n = 18), PDR (n = 25), BRVO (n = 7) and cataract (n = 22). The levels of VEGF-A, IL-8 and EPO were measured with ELISA. Results: Aqueous VEGF-A significantly decreased after anti-VEGF and PRP, from 983.79 +/- 821.16 pg/ml in the NVG group (n = 11) to 256.50 +/- 51.14 pg/ml in the stable NVG group (n = 24) (P = .015). Aqueous VEGF-A in stable NVG group (256.50 +/- 51.14 pg/ml, n = 24) was significantly higher (ANOVA, P < .001) than in CRVO (212.10 +/- 19.84 pg/ml, n = 7, P = .017), NPDR (221.18 +/- 38.21 pg/ml, n = 14, P = .015), BRVO (213.14 +/- 48.50 pg/ml, n = 6, P = .028) and cataract group (185.30 +/- 34.35 pg/ml, n = 22, P < .001). Aqueous IL-8 in stable NVG group (74.82 +/- 10.78 pg/ml, n = 24) was significantly higher (ANOVA, P < .001) than in CRVO (65.19 +/- 15.34 pg/ml, n = 11, P = .032) and cataract group (54.11 +/- 12.28 pg/ml, n = 22, P < .001). Aqueous EPO in stable NVG group (17.48 +/- 3.02 pg/ml, n = 24) was significantly higher (ANOVA, P < .001) than in BRVO (14.98 +/- 2.57 pg/ml, n = 7, P = .034) and cataract group (13.50 +/- 2.65 pg/ml, n = 22, P < .001). Aqueous concentrations of VEGF-A and IL-8 correlated positively with IOP (r = 0.413, P < .001, r = 0.349, P < .001, respectively, r = correlation coefficient). VEGF-A correlated positively with IL-8 and EPO (P < .001, P = .002, respectively). IL-8 correlated positively with EPO (P < .001). Conclusions: The aqueous levels of VEGF-A, IL-8 and EPO in NVG patients with stable iris neovascularization, who had received PRP and anti-VEGF, were still significantly higher than in control groups with some retinal ischemic conditions.