Downregulation of long noncoding RNA DGCR5 contributes to the proliferation, migration, and invasion of cervical cancer by activating Wnt signaling pathway
单位：[1]Department of Obstetrics & Gynecology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China临床科室妇产科妇产科首都医科大学附属北京友谊医院[2]Department of Gynecology Minimally Invasive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China[3]Department of Pathology, Huai’an Maternal and Child Health Care Center of Jiangsu Province Affiliated Hospital of Yangzhou University, Huai'an, China[4]Department of Gynaecology, Huai’an Maternal and Child Health Care Center of Jiangsu Province Affiliated Hospital of Yangzhou University, Huai'an, China
Accumulating research works have reported that long noncoding RNAs (lncRNAs) are involved in various cancers, including cervical cancer. LncRNA DGCR5 has been identified in many cancers. However, the biological role of DGCR5 in cervical cancer remains barely known. We aimed to investigate the biological function of DGCR5 in cervical cancer progression. Here, in our current study, we observed that DGCR5 was downregulated in human cervical cancer cell lines (MS751, SiHa, HeLa, and HT-3) compared with the primary normal cervical squamous cells (NCSC1 and NCSC2). Then, DGCR5 was restrained by transfection with lenti-virus-short hairpin RNA (LV-shRNA) while induced by LV-DGCR5 in HeLa and C33A cells. Silence of DGCR5 obviously induced cervical cancer cell viability and cell proliferation. Reversely, upregulation of DGCR5 inhibited HeLa and C33A cell survival and proliferation. Furthermore, silencing of DGCR5 increased cervical cancer cell colony formation ability and decreased cell apoptosis, whereas its overexpression exhibited an opposite process. Moreover, DGCR5 suppressed migration and invasion capacity of cervical cancer cells. The Wnt signaling is integral in numerous biological processes. Here, we found that Wnt signaling was strongly activated in cervical cancer cells. Downregulation of DGCR5 contributed to cervical cancer progression by activating Wnt signaling. Subsequently, in vivo animal models were used to confirm that DGCR5 suppressed cervical cancer via targeting Wnt signaling. In conclusion, we reported that DGCR5 was involved in cervical cancer progression via modulating the Wnt pathway.
第一作者单位：[1]Department of Obstetrics & Gynecology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[3]Department of Pathology, Huai’an Maternal and Child Health Care Center of Jiangsu Province Affiliated Hospital of Yangzhou University, Huai'an, China[4]Department of Gynaecology, Huai’an Maternal and Child Health Care Center of Jiangsu Province Affiliated Hospital of Yangzhou University, Huai'an, China[*1]Department of Pathology, Huai’an Maternal and Child Health Care Center of Jiangsu Province Affiliated Hospital of Yangzhou University, 223002 Huai’an, China.
推荐引用方式(GB/T 7714)：
Liu Yun,Chang Yue,Lu Shuxiong,et al.Downregulation of long noncoding RNA DGCR5 contributes to the proliferation, migration, and invasion of cervical cancer by activating Wnt signaling pathway[J].JOURNAL of CELLULAR PHYSIOLOGY.2019,234(7):11662-11669.doi:10.1002/jcp.27825.
APA：
Liu, Yun,Chang, Yue,Lu, Shuxiong&Xiang, Yuan-Yuan.(2019).Downregulation of long noncoding RNA DGCR5 contributes to the proliferation, migration, and invasion of cervical cancer by activating Wnt signaling pathway.JOURNAL of CELLULAR PHYSIOLOGY,234,(7)
MLA：
Liu, Yun,et al."Downregulation of long noncoding RNA DGCR5 contributes to the proliferation, migration, and invasion of cervical cancer by activating Wnt signaling pathway".JOURNAL of CELLULAR PHYSIOLOGY 234..7(2019):11662-11669
