单位：[1]Department of Comprehensive Surgery, Medical and Health Center, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of China临床科室医保中心医保外科首都医科大学附属北京友谊医院[2]East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA[3]Human Performance Laboratory, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA[4]Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA[5]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing, People’s Republic of China临床科室国家中心普外分中心普外五科（综合普外科）肿瘤中心首都医科大学附属北京友谊医院[6]Department of Physiology, East Carolina University, Greenville, North Carolina, USA
Vertical sleeve gastrectomy (VSG) is an effective surgery to treat obesity and diabetes. However, the direct effect of VSG on metabolic functions is not fully understood. We aimed to investigate if alterations in hypothalamic neurons were linked with perturbations in liver metabolism after VSG in an energy intake-controlled obese mouse model. CS7BL/6 and hrNPY-GFP reporter mice received HFD for 12 weeks and were then divided into three groups: Sham (ad lib), Sham (pair-fed) with VSG and VSG. Food intake was measured daily, and blood glucose levels were measured before and after the study. Energy expenditure and body composition were determined. Serum parameters, liver lipid and glycogen contents were measured and gene/protein expression were analyzed. Hypothalamic POMC, AgRP/NPY and tyrosine hydroxylase-expressing neurons were counted. The following results were obtained. VSG reduced body weight gain and adiposity induced by HFD, increased energy expenditure independent of energy intake. Fed and fasted blood glucose levels were reduced in the VSG group. While serum active GLP-1 level was increased, the active ghrelin and triglycerides levels were decreased along with improved insulin resistance in VSG group. Liver lipid accumulation, glycogen content and gluconeogenic gene expression were reduced in the VSG group. In the hypothalamus, TH-expressing neuron population was decreased, and the POMC-expressing neuron population was increased in the VSG group. In conclusion, our data suggest that VSG improves metabolic symptoms by increasing energy expenditure and lowering lipid and glycogen contents in the liver. These physiological alterations are possibly related to changes in hypothalamic neuron populations.
第一作者单位：[1]Department of Comprehensive Surgery, Medical and Health Center, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of China[2]East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA[3]Human Performance Laboratory, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA
通讯作者：
通讯机构：[2]East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA[3]Human Performance Laboratory, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA[4]Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA[6]Department of Physiology, East Carolina University, Greenville, North Carolina, USA
推荐引用方式(GB/T 7714)：
Li Peixin,Raoz Zhijian,Laing Brenton Thomas,et al.Vertical sleeve gastrectomy improves liver and hypothalamic functions in obese mice[J].JOURNAL of ENDOCRINOLOGY.2019,241(2):135-147.doi:10.1530/JOE-18-0658.
APA：
Li, Peixin,Raoz, Zhijian,Laing, Brenton Thomas,Bunner, Wyatt,Landry, Taylor...&Huang, Hu.(2019).Vertical sleeve gastrectomy improves liver and hypothalamic functions in obese mice.JOURNAL of ENDOCRINOLOGY,241,(2)
MLA：
Li, Peixin,et al."Vertical sleeve gastrectomy improves liver and hypothalamic functions in obese mice".JOURNAL of ENDOCRINOLOGY 241..2(2019):135-147
