单位：[1]Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China[2]Graduate School of Peking Union Medical College, Beijing 100730, China[3]Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing 100043, China[4]Stanford Genome Technology Center, Stanford University, Palo Alto CA 94003, USA[5]HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA[6]The First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong 515041, China[7]Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属北京友谊医院[8]Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100026, China
Preeclampsia (PE) is one of the major causes of maternal and perinatal mortality worldwide. This study aimed to determine the immunological characteristics of PE patients and normal pregnancy at the T cell receptor betachain (TRB) level by using high-throughput sequencing. High-throughput sequencing was performed to analyze the expression of TRB-CDR3 in circulating T cells. T cells were isolated from 36 healthy pregnant women, 24 patients with severe PE, and 18 patients with moderate PE. Rearranged mRNA sequences were assigned to their germline V, D, and J counterparts, and translated into proper amino acids by the IMGT database. In general, PE samples had more TRB-CDR3 reads and types than those of normal pregnant woman in the circulation, but the mean number of TRB-CDR3 reads and unique TRB-CDR3 reads in severe group was lower than that in the moderate group. In PE patients, the V7_9 and V20_1 gene loci were more prevalent than in healthy pregnant women. In addition, 4 kinds of TRB-CDR3 peptides were found to be highly relevant to the pathogenesis of PE. Of them, peptides matched to herpes simplex virus antigen-specific T cells were much lower in PE samples.
基金：
National Key Research and Development Program [2016YFC1000300, 2016YFC1000307]; [0102012DFB30130]
第一作者单位：[1]Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China[2]Graduate School of Peking Union Medical College, Beijing 100730, China[3]Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing 100043, China[4]Stanford Genome Technology Center, Stanford University, Palo Alto CA 94003, USA[*1]Graduate School of Peking Union Medical College, Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China.
推荐引用方式(GB/T 7714)：
Guo Changlong,Cai Ruikun,Cao Xiaofang,et al.Deep targeted sequencing reveals the diversity of TRB-CDR3 repertoire in patients with preeclampsia[J].HUMAN IMMUNOLOGY.2019,80(10):848-854.doi:10.1016/j.humimm.2019.04.003.
APA：
Guo, Changlong,Cai, Ruikun,Cao, Xiaofang,Yang, Ying,Wang, Qidi...&Ma, Xu.(2019).Deep targeted sequencing reveals the diversity of TRB-CDR3 repertoire in patients with preeclampsia.HUMAN IMMUNOLOGY,80,(10)
MLA：
Guo, Changlong,et al."Deep targeted sequencing reveals the diversity of TRB-CDR3 repertoire in patients with preeclampsia".HUMAN IMMUNOLOGY 80..10(2019):848-854
