单位：[1]School of Material Science and Engineering, Changzhou University, Changzhou, Jiangsu 213164, PR China[2]Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China[3]Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, 100029, PR China[4]State Key Laboratory of Bioactive Seaweed Substances, Qingdao Brightmoon Seaweed Group Co Ltd, Qingdao, 266400, PR China
Chitosan and alginate have been considered to be promising natural polymers for biomedical application because of their biocompatibility, biodegradability and similarity to macromolecules recognized by human body, which are extensively used in wounds and burns management. Here a sponge-like polyelectrolyte-complex multilayer membrane (PCMM) was prepared via freeze-drying method. The attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and field emission scanning electron microscopy (FE-SEM) analysis revealed that chitosan/alginate layer exhibited gradient porous structure and interpenetrating polymer networks in PCMM. The porosity and swelling ratio of PCMM were evaluated. The results indicate that the prepared PCMM shows porosity up to 90% and equilibrium swelling ratio up to 22.4 in deionized water as well as 21.5 in ion-containing solution. Antibacterial activity evaluated in vitro shows the PCMM appears obviously antibacterial activity towards E. coli. Cytotoxicity study evaluated in vitro illustrates the nontoxic nature of the PCMM, and it is good for the cell attachment, growth, and proliferation. These results strongly support the possibility of using this novel PCMM for wound care.
基金：
Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances; China-Japan Friendship Hospital Youth Science and Technology Excellence Project [2015-QNYC-B-06]
第一作者单位：[1]School of Material Science and Engineering, Changzhou University, Changzhou, Jiangsu 213164, PR China[2]Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China
通讯作者：
推荐引用方式(GB/T 7714)：
Sun Wenwen,Chen Guangkai,Wang Fahe,et al.Polyelectrolyte-complex multilayer membrane with gradient porous structure based on natural polymers for wound care[J].CARBOHYDRATE POLYMERS.2018,181:183-190.doi:10.1016/j.carbpol.2017.10.068.
APA：
Sun, Wenwen,Chen, Guangkai,Wang, Fahe,Qin, Yimin,Wang, Zai...&Ma, Guiping.(2018).Polyelectrolyte-complex multilayer membrane with gradient porous structure based on natural polymers for wound care.CARBOHYDRATE POLYMERS,181,
MLA：
Sun, Wenwen,et al."Polyelectrolyte-complex multilayer membrane with gradient porous structure based on natural polymers for wound care".CARBOHYDRATE POLYMERS 181.(2018):183-190
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