单位：[1]Department of Urology, Friendship Hospital, Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Capital Medical University, Beijing, China临床科室泌尿外科泌尿外科首都医科大学附属北京友谊医院[2]Department of Urology, Beijing Luhe Hospital, Capital Medical University, Beijing, China[3]Department of Pathology, Capital Medical University, Beijing, China
Transforming growth factor--induced (TGFBI) is considered to be a vital gene in several carcinomas. In this study we determined the effect of TGFBI on the proliferative and metastatic potential of human urothelial carcinoma (UC) cells as well as its mRNA and protein expression, which were detected by RT-PCR and western blot, respectively. UC cell proliferation was analyzed by WST-1 assay and Hoechst 33258 staining. The effect of TGFBI on UC cell metastasis was analyzed using adhesion, migration and invasion assays. We found that TGFBI increased the proliferation of UC cells. Moreover, TGFBI enhanced the adhesion, migration and invasion of UC cells by upregulating MMP-2, MMP-9 and calpain-2 expression. We evaluated the effect of Epirubicin (EPI) on the regulation of TGFBI expression and found that TGFBI acts as a downstream target of EPI and is suppressed by EPI in UC cells. EPI is more effective in inhibiting the proliferation and metastasis of UC cells with high TGFBI expression. This study demonstrates that TGFBI might lead to tumorigenesis and progression of UC and those cells with high TGFBI expression may be vulnerable to relapse. EPI could prove to be a therapeutic option in patients with high TGFBI expressing UC cells.
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201604]
第一作者单位：[1]Department of Urology, Friendship Hospital, Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[3]Department of Pathology, Capital Medical University, Beijing, China[*1]Department of Pathology, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Shang Donghao,Song Bo,Liu Yuting.Epirubicin suppresses proliferative and metastatic potential by downregulating transforming growth factor--induced expression in urothelial carcinoma[J].CANCER SCIENCE.2018,109(4):980-987.doi:10.1111/cas.13403.
APA：
Shang, Donghao,Song, Bo&Liu, Yuting.(2018).Epirubicin suppresses proliferative and metastatic potential by downregulating transforming growth factor--induced expression in urothelial carcinoma.CANCER SCIENCE,109,(4)
MLA：
Shang, Donghao,et al."Epirubicin suppresses proliferative and metastatic potential by downregulating transforming growth factor--induced expression in urothelial carcinoma".CANCER SCIENCE 109..4(2018):980-987
