CC Chemokine Ligand 18 Promotes Cell Proliferation and Metastasis of Urothelial Carcinoma via Activating PI3K/mTOR Signaling in Patient with Renal Transplantation
单位：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室泌尿外科泌尿外科首都医科大学附属北京友谊医院[2]Department of Urology, Beijing Luhe Hospital, Capital Medical University, Beijing, China
Objective: The study aimed to investigate the role of chemokine (C-C motif) ligand 18 (CCL18) in the progression of urothelial carcinoma (UC) after renal transplants (RT). Methods: Tissues were collected from 60 patients with UC in which 24 were after RT. The tissues were all obtained from patients after radical cystectomy. Expression levels of CCL18 were examined by immunohistochemistry, RT-polymerase chain reaction (RT-PCR), and quantitative RT-PCR, respectively. WST-1 assay was performed to determine proliferative capacity of UC cells. Migration and invasion of UC cells were analyzed by the using of chemotaxis and Transwell system. Western blotting was applied to assess the activation of PI3K-mTOR (Mammalian Target of Rapamycin) signaling. Results: Compared to normal urothelial tissues, the expression levels of CCL18 were significantly increased in tumors of UC, and even more in tumors of UC after RT. Ectopic expression of CCL18 efficiently enhanced the proliferation of UC cells and moreover, exacerbated the migration and invasion of UC cells. Furthermore, overexpressed CCL18 increased the phosphorylation of PI3K, Akt, and mTOR in UC cells, suggesting the hyperactivation of PI3K/Akt/mTOR signaling. Conclusions: The results demonstrate the critical role of CCL18 in the progression of UC, especially in receipt with RT. Our study indicates that CCL18 could serve as a biomarker and therapeutic target in diagnosis of recipients with UC after RT. (c) 2018 S. Karger AG, Basel
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201604]
第一作者单位：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China[2]Department of Urology, Beijing Luhe Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China[*1]Department of Urology, Beijing Friendship Hospital Capital Medical University, 95 Yongan Road Beijing 100050 (China)
推荐引用方式(GB/T 7714)：
Song Bo,Shang Donghao,Kong Guangqi,et al.CC Chemokine Ligand 18 Promotes Cell Proliferation and Metastasis of Urothelial Carcinoma via Activating PI3K/mTOR Signaling in Patient with Renal Transplantation[J].UROLOGIA INTERNATIONALIS.2018,101(4):450-458.doi:10.1159/000492180.
APA：
Song Bo,Shang Donghao,Kong Guangqi&Tian Ye.(2018).CC Chemokine Ligand 18 Promotes Cell Proliferation and Metastasis of Urothelial Carcinoma via Activating PI3K/mTOR Signaling in Patient with Renal Transplantation.UROLOGIA INTERNATIONALIS,101,(4)
MLA：
Song Bo,et al."CC Chemokine Ligand 18 Promotes Cell Proliferation and Metastasis of Urothelial Carcinoma via Activating PI3K/mTOR Signaling in Patient with Renal Transplantation".UROLOGIA INTERNATIONALIS 101..4(2018):450-458
