单位：[1]Institute and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei 230032, Anhui, China.[2]Department of Dermatology, China-Japan Friendship Hospital, Beijing 100029, China.[3]Graduate School, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.[4]Department of Biochemistry, University of New Mexico, Albuquerque, NM 87131, USA.[5]Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University, Hefei 230601, Anhui, China.[6]Hebrew SeniorLife Institute for Aging Research and Harvard Medical School, Boston, MA 02131, USA.[7]Molecular and Integrative Physiological Sciences, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.[8]Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.[9]Institute and Department of Dermatology, Huashan Hospital of Fudan University, Shanghai 200040, China.[10]Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.
BackgroundPsoriasis (Ps) is a common chronic inflammatory skin disease. The keratinocytes of psoriatic skin defy normal apoptosis and exhibit active cell proliferation. Aberrant DNA methylation (DNAm) has been suggested relevant through regulating the expression of Ps susceptibility genes. However, it is unclear whether the biological age inferred from DNA methylome is affected.ResultsTo address the above issue, we applied a recently developed methylation clock model to our Chinese Han population dataset, which includes DNAm data of 114 involved psoriatic skin tissues (PP) and 41 uninvolved psoriatic skin tissues (PN) from Ps patients, and 62 normal skin tissues (NN) from health controls. We first confirmed the applicability of the clock in PN and NN. We then showed that PP samples have largely unchanged DNAm age, and that no association was observed between available clinical features and DNAm age acceleration. Examination of genome-wide CpGs yielded age-associated CpGs with concordant age-association coefficients among the three groups, which was also supported by an external dataset. We also interestingly observed two clock CpGs differentially methylated between PP and PN.ConclusionsOverall, our results suggest no significant alteration in DNAm age in PN and PP. Therefore, the increase in keratinocyte proliferation and alteration in DNAm caused by Ps may not affect the biological age of psoriatic skin tissue.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81502713, 81573033, 81872516]; Grant for Scientific Research of BSKY from Anhui Medical University [XJ201634]; Key Program of Outstanding Talents of Anhui Province [gxyqZD2017028]; Fundamental Research Funds for the Central Universities, ChinaFundamental Research Funds for the Central Universities [3332018182]; Innovation Fund for Graduate Students, Peking Union Medical College, China [2018-1002-01-26]
第一作者单位：[1]Institute and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei 230032, Anhui, China.[2]Department of Dermatology, China-Japan Friendship Hospital, Beijing 100029, China.[3]Graduate School, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.[6]Hebrew SeniorLife Institute for Aging Research and Harvard Medical School, Boston, MA 02131, USA.[7]Molecular and Integrative Physiological Sciences, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.[8]Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
共同第一作者：
通讯作者：
通讯机构：[2]Department of Dermatology, China-Japan Friendship Hospital, Beijing 100029, China.[3]Graduate School, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.
推荐引用方式(GB/T 7714)：
Changbing Shen,Leilei Wen,Randy Ko,et al.DNA methylation age is not affected in psoriatic skin tissue[J].CLINICAL EPIGENETICS.2018,10:doi:10.1186/s13148-018-0584-y.
APA：
Changbing Shen,Leilei Wen,Randy Ko,Jing Gao,Xue Shen...&Fusheng Zhou.(2018).DNA methylation age is not affected in psoriatic skin tissue.CLINICAL EPIGENETICS,10,
MLA：
Changbing Shen,et al."DNA methylation age is not affected in psoriatic skin tissue".CLINICAL EPIGENETICS 10.(2018)
