单位：[1]State Key Laboratory for Infectious Disease Prevention and Control, National Institute forCommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing, China, 102206[2]Collaborative Innovation Center for Diagnosis and Treatment of InfectiousDiseases, Hangzhou, China, 310003[3]Key Laboratory of Surveillance and Early-warning onInfectious Disease, Division of Infectious Disease, Chinese Center for Disease Control and Prevention,Beijing 102206, China[4]Department of clinical laboratory, China-Japan Friendship Hospital, Beijing100029, China[5]Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University[6]Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100011, China
Clostridium difficile, the etiological agent of Clostridium difficile infection (CDI), is a gram-positive, spore-forming bacillus that is responsible for similar to 20% of antibiotic-related cases of diarrhea and nearly all cases of pseudomembranous colitis. Previous data have shown that a substantial proportion (11%) of the C. difficile genome consists of mobile genetic elements, including seven conjugative transposons. However, the mechanism underlying the formation of a mosaic genome in C. difficile is unknown. The type-IV secretion system (T4SS) is the only secretion system known to transfer DNA segments among bacteria. We searched genome databases to identify a candidate T4SS in C. difficile that could transfer DNA among different C. difficile strains. All T4SS gene clusters in C. difficile are located within genomic islands (GIs), which have variable lengths and structures and are all conjugative transposons. During the horizontal-transfer process of T4SS GIs within the C. difficile population, the excision sites were altered, resulting in different short-tandem repeat sequences among the T4SS GIs, as well as different chromosomal insertion sites and additional regions in the GIs.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81301402]; 863 ProjectNational High Technology Research and Development Program of China [2014AA021505, 2013ZX10004221, 2013ZX10004-101-002]
第一作者单位：[1]State Key Laboratory for Infectious Disease Prevention and Control, National Institute forCommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing, China, 102206[2]Collaborative Innovation Center for Diagnosis and Treatment of InfectiousDiseases, Hangzhou, China, 310003
共同第一作者：
通讯作者：
通讯机构：[1]State Key Laboratory for Infectious Disease Prevention and Control, National Institute forCommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing, China, 102206[2]Collaborative Innovation Center for Diagnosis and Treatment of InfectiousDiseases, Hangzhou, China, 310003[5]Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University[6]Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100011, China
推荐引用方式(GB/T 7714)：
Wen Zhang,Ying Cheng,Pengcheng Du,et al.Genomic study of the Type IVC secretion system in Clostridium difficile: understanding C. difficile evolution via horizontal gene transfer[J].GENOME.2017,60(1):8-16.doi:10.1139/gen-2016-0053.
APA：
Wen Zhang,Ying Cheng,Pengcheng Du,Yuanyuan Zhang,Hongbing Jia...&Jinxing Lu.(2017).Genomic study of the Type IVC secretion system in Clostridium difficile: understanding C. difficile evolution via horizontal gene transfer.GENOME,60,(1)
MLA：
Wen Zhang,et al."Genomic study of the Type IVC secretion system in Clostridium difficile: understanding C. difficile evolution via horizontal gene transfer".GENOME 60..1(2017):8-16
