Ezrin-Radixin-Moesin Binding Phosphoprotein 50 (EBP50) Suppresses the Metastasis of Breast Cancer and HeLa Cells by Inhibiting Matrix Metalloproteinase-2 Activity
单位：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China临床科室泌尿外科泌尿外科首都医科大学附属北京友谊医院[2]Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China[3]Beijing Key Laboratory for Tumor Invasion and Metastasis, Beijing International CooperationBase for Science and Technology on China-UK Cancer Research, Beijing, P.R. China
Background: Expression of ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) is correlated with human breast and cervical cancer development, but its effects on the metastasis of breast and cervical cancer and the underlying mechanism are not fully understood. Materials and Methods: In this study, EBP50 was overexpressed in MDA-MB-231 breast cancer and HeLa cervical cancer cells; moreover, EBP50 was knocked-down in MCF-7 breast cancer cells and HeLa cells. Metastasis-related ability and matrix metalloproteinase-2 (MMP-2) activity of these cells were investigated. Results: Cell adhesion, wound-healing and invasion were significantly suppressed in EBP50-overexpressing cells. Contrarily, EBP50-knockdown promoted cell adhesion, wound healing and invasion. EBP50 overexpression inhibited MMP-2 activity, and the knockdown of EBP50 promoted the activity of MMP-2, suggesting that EBP50 inhibited cell metastasis via suppression of MMP-2 activity. Conclusion: Our work reveals the anti-metastatic effect and a new mechanism of EBP50 action in breast and cervical cancer cells.
基金：
National Natural Science Foundation of the People's Republic of ChinaNational Natural Science Foundation of China (NSFC) [81372739, 81672521]; Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions [CITTCD201304187]; Basic-Clinic Cooperation Project of Capital Medical University [14JL-L03]; Beijing Friendship Hospital [yyqdkt2015-14]
第一作者单位：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China[3]Beijing Key Laboratory for Tumor Invasion and Metastasis, Beijing International CooperationBase for Science and Technology on China-UK Cancer Research, Beijing, P.R. China[*1]Department of Biochemistry and Molecular Biology, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing 100069, P.R. China
推荐引用方式(GB/T 7714)：
Wang Lei,Qi Yijun,Xiong Ying,et al.Ezrin-Radixin-Moesin Binding Phosphoprotein 50 (EBP50) Suppresses the Metastasis of Breast Cancer and HeLa Cells by Inhibiting Matrix Metalloproteinase-2 Activity[J].ANTICANCER RESEARCH.2017,37(8):4353-4360.doi:10.21873/anticanres.11829.
APA：
Wang, Lei,Qi, Yijun,Xiong, Ying,Peng, Zhiqiang,Ma, Qiang...&Zheng, Junfang.(2017).Ezrin-Radixin-Moesin Binding Phosphoprotein 50 (EBP50) Suppresses the Metastasis of Breast Cancer and HeLa Cells by Inhibiting Matrix Metalloproteinase-2 Activity.ANTICANCER RESEARCH,37,(8)
MLA：
Wang, Lei,et al."Ezrin-Radixin-Moesin Binding Phosphoprotein 50 (EBP50) Suppresses the Metastasis of Breast Cancer and HeLa Cells by Inhibiting Matrix Metalloproteinase-2 Activity".ANTICANCER RESEARCH 37..8(2017):4353-4360
