Reduced EBP50 expression levels are correlated with unfavorable clinicopathological features of extrahepatic bile duct carcinoma and promote the proliferation and migration of QBC939 cells
单位：[1]Department of Radiology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001[2]Beijing Key Laboratory for Tumor Invasion and Metastasis, Cancer Institute of Capital Medical University[3]Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069[4]Department of Pathology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001[5]Department of General Surgery, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001[6]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China临床科室泌尿外科泌尿外科首都医科大学附属北京友谊医院
The present study aimed to clarify the association between ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) expression level and the tumor phenotype and clinicopathological features of extrahepatic bile duct carcinoma. Tissue samples from patients with extrahepatic bile duct carcinoma (54 cases) and patients with normal bile duct epithelia from gallbladder of cholecystitis (20 cases) were collected, and immunohistochemical staining was used to detect the expression levels of EBP50 in these tissues. In addition, small interfering (si)RNA-EBP50 was used to knock down the expression of EBP50 in the QBC939 human cholangiocarcinoma (CC) cell line. The effect of EBP50 expression on QBC939 cell proliferation and migration was analyzed using the Cell Counting kit-8 and wound healing assays, respectively. EBP50 expression was significantly downregulated in CC tissue samples (P< 0.01), with low EBP50 expression levels positively correlated with a high pathological stage and a poor differentiation degree (P< 0.01 and P< 0.001, respectively). EBP50 expression in QBC939 cells was knocked down by <= 80% using siRNA-EBP50, and EBP50 knockdown significantly promoted QBC939 cell proliferation, as compared with the vector control cells (P= 0.04). EBP50 knockdown also significantly enhanced the wound healing ability of QBC939 cells (P= 0.02). These results demonstrated that EBP50 expression levels are significantly correlated with a malignant phenotype in patients with CC, and decreased expression levels of EBP50 may promote CC cell proliferation and migration. These findings provide insight into novel potential diagnostic and therapeutic approaches for patients with CC.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81471652]; Postdoctoral Science Foundation of ChinaChina Postdoctoral Science Foundation [2013M541208]; Natural Science Foundation of Shanxi provinceNatural Science Foundation of Shanxi Province [2014011037-3]; Tumor Invasion and Metastasis Research Foundation of Beijing Key Laboratory [2015ZLQX04]
第一作者单位：[1]Department of Radiology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001[2]Beijing Key Laboratory for Tumor Invasion and Metastasis, Cancer Institute of Capital Medical University
共同第一作者：
通讯作者：
通讯机构：[1]Department of Radiology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001[6]Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China[*1]Department of Radiology, The First Hospital of Shanxi Medical University, 85 Jiefang South Road, Taiyuan, Shanxi 030001, P.R. China[*2]Department of Urology, Beijing Friendship Hospital, Capital Medical University, 95 Yong An Road, Xi Cheng, Beijing 100050, P.R. China
推荐引用方式(GB/T 7714)：
Feng Duiping,Xiong Ying,Peng Zhiqiang,et al.Reduced EBP50 expression levels are correlated with unfavorable clinicopathological features of extrahepatic bile duct carcinoma and promote the proliferation and migration of QBC939 cells[J].ONCOLOGY LETTERS.2017,13(4):2758-2764.doi:10.3892/ol.2017.5789.
APA：
Feng, Duiping,Xiong, Ying,Peng, Zhiqiang,Ma, Qiang,Tao, Tao...&Zhang, Hui.(2017).Reduced EBP50 expression levels are correlated with unfavorable clinicopathological features of extrahepatic bile duct carcinoma and promote the proliferation and migration of QBC939 cells.ONCOLOGY LETTERS,13,(4)
MLA：
Feng, Duiping,et al."Reduced EBP50 expression levels are correlated with unfavorable clinicopathological features of extrahepatic bile duct carcinoma and promote the proliferation and migration of QBC939 cells".ONCOLOGY LETTERS 13..4(2017):2758-2764
