单位：[1]Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing 100730, China[2]Department of Geriatrics, Beijing Hospital, Beijing 100730, China[3]Department of Laboratory Medicine, Beijing Hospital, Beijing 100730, China[4]Department of Hospital Infection Control and Management, Beijing Hospital, Beijing 100730, China[5]Department of Respiratory Medicine, Chinese-Japanese Friendship Hospital, Beijing 100029, China
Objective To gain greater insight into the prevalence drug resistant profiles of M. abscessus from a general hospital in Beijing, China. Methods Partial gene sequencing of 16S, hsp65, and rpoB were used to distinguish the species of NTM isolates. All strains identified as M. abscessus were further enrolled in the drug susceptibility testing by using broth microdilution method. Results We found that M. avium complex was the most frequent NTM organism, accounting for 54.1% (33/61) of all isolates. Behind MAC, the second most common organisms were M. abscessus (22 out of 61, 36.1%). Average rates of resistance were 4.5% for AMK, 9.1% for LZD, and 13.6% for CLA, respectively. In contrast, resistance to LEV (17/22, 77.3%), IMI (9/22, 40.9%), and SMX (10/22, 45.5%) was noted in more than 40% of M. abscessus isolates. DNA sequencing revealed that all the CLA-resistant isolates harbored nucleotide substitutions in position 2058 (1/3, 33.3%) or 2059 (2/3, 66.7%) of 23S rRNA. Conclusion In conclusion, our data demonstrated that M. intracellulare and M. abscessus were the most common NTM species in the general hospital of Beijing. CLA, AMK, LZD showed promising activity, where as LEV, IMI, and SMX exhibited poor activity against M. abscessus in vitro.
基金：
National Key Technology Research and Development Program; ChinaNational Key Technology R&D Program [2012BAI05B02]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81400037]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区环境科学与生态学
小类|4 区环境科学4 区公共卫生、环境卫生与职业卫生
最新[2025]版：
大类|4 区医学
小类|4 区环境科学4 区公共卫生、环境卫生与职业卫生
JCR分区：
出版当年[2014]版：
Q2ENVIRONMENTAL SCIENCESQ2PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
最新[2023]版：
Q2ENVIRONMENTAL SCIENCESQ2PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
第一作者单位：[1]Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing 100730, China[4]Department of Hospital Infection Control and Management, Beijing Hospital, Beijing 100730, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
LI Yan Ming,TONG Xun Liang,XU Hong Tao,et al.Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China[J].BIOMEDICAL and ENVIRONMENTAL SCIENCES.2016,29(2):85-90.doi:10.3967/bes2016.009.
APA：
LI Yan Ming,TONG Xun Liang,XU Hong Tao,JU Yang,CAI Meng&WANG Chen.(2016).Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China.BIOMEDICAL and ENVIRONMENTAL SCIENCES,29,(2)
MLA：
LI Yan Ming,et al."Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China".BIOMEDICAL and ENVIRONMENTAL SCIENCES 29..2(2016):85-90
