Identification of downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate signal pathway in pulmonary hypertension
单位：[1]Key Laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing, China[2]Department of Respiratory and Critical Care Medicine, Beijing Hospital, Ministry of Health, Beijing, China[3]Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Ministry of Health, Beijing, China[4]National Clinical Research Center of Respiratory Medicine, Beijing, China
Objective To investigate the downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate (NO-sGC-cGMP) signal pathway and their possible roles in the pathogenesis of pulmonary hypertension (PH). Methods Digital gene expression tag profiling was performed to identify genes that are differentially expressed after activation of the NO-sGC-cGMP signal pathway in human pulmonary artery smooth muscles cells using 8-bromo-cyclic guanosine monophosphate, BAY 41-2272 and BAY 60-2770. Results were confirmed using real-time polymerase chain reaction. Gene ontology and signal transduction network analyses were also performed. Results A number of genes were differentially expressed, including MMP1, SERPINB2, GREM1 and IL8. A total of 68 gene ontology terms and seven pathways were found to be associated with these genes. Most of these genes are involved in cell proliferation, cell migration and apoptosis, which may contribute to the pathological pulmonary vascular remodelling in PH. Conclusion These results may provide new insights into the molecular mechanisms of PH.
基金：
National High Technology Research and Development ProgramNational High Technology Research and Development Program of China [2012AA02A511]; National Department Public Benefit Research Foundation [201302008]; National Key Technology Research and Development ProgramNational Key Technology R&D Program [2013BAI09B00]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81400036]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
JCR分区：
出版当年[2014]版：
Q3MEDICINE, RESEARCH & EXPERIMENTALQ3PHARMACOLOGY & PHARMACY
最新[2023]版：
Q4MEDICINE, RESEARCH & EXPERIMENTALQ4PHARMACOLOGY & PHARMACY
第一作者单位：[1]Key Laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing, China
通讯作者：
通讯机构：[3]Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Ministry of Health, Beijing, China[4]National Clinical Research Center of Respiratory Medicine, Beijing, China[*1]China-Japan Friendship Hospital, Ministry of Health, 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
推荐引用方式(GB/T 7714)：
Lihui Zou,Xiaomao Xu,Zhenguo Zhai,et al.Identification of downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate signal pathway in pulmonary hypertension[J].JOURNAL of INTERNATIONAL MEDICAL RESEARCH.2016,44(3):508-519.doi:10.1177/0300060516636751.
APA：
Lihui Zou,Xiaomao Xu,Zhenguo Zhai,Ting Yang,Junhua Jin...&Chen Wang.(2016).Identification of downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate signal pathway in pulmonary hypertension.JOURNAL of INTERNATIONAL MEDICAL RESEARCH,44,(3)
MLA：
Lihui Zou,et al."Identification of downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate signal pathway in pulmonary hypertension".JOURNAL of INTERNATIONAL MEDICAL RESEARCH 44..3(2016):508-519
