Cell landscape atlas for patients with chronic thromboembolic pulmonary hypertension after pulmonary endarterectomy constructed using single-cell RNA sequencing
单位：[1]Medical Research Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[2]Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Institute of Respiratory Medicine, Beijing 100020, China[3]Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China[4]Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[5]Department of Echocardiography, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[6]Department of Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[7]Department of Interventional Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[8]Department of Pathology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院[9]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China[10]Department of Radiology, China-Japan Friendship Hospital, Beijing 100029, China[11]Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China北京朝阳医院
This study aimed to construct an atlas of the cell landscape and comprehensively characterize the cellular repertoire of the pulmonary endarterectomized tissues of patients with chronic thromboembolic pulmonary hypertension (CTEPH). Five pulmonary endarterectomized tissues were collected. 10x Genomics single-cell RNA sequencing was performed, followed by the identification of cluster marker genes and cell types. Gene Ontology (GO) enrichment analysis was conducted. Seventeen cell clusters were characterized, corresponding to 10,518 marker genes, and then classified into eight cell types, including fibroblast/smooth muscle cell, endothelial cell, T cell/NK cell, macrophage, mast cell, cysteine rich secretory protein LCCL domain containing 2 (CRISPLD2)+ cell, cancer stem cell, and undefined. The specific marker genes of fibroblast/smooth muscle cell, endothelial cell, T cell/NK cell, macrophage, mast cell, and cancer stem cell were significantly enriched for multiple functions associated with muscle cell migration, endothelial cell migration, T cell activation, neutrophil activation, erythrocyte homeostasis, and tissue remodeling, respectively. No functions were significantly enriched for the marker gene of CRISPLD2+ cell. Our study, for the first time, provides an atlas of the cell landscape of the pulmonary endarterectomized tissues of CTEPH patients at single-cell resolution, which may serve as a valuable resource for further elucidation of disease pathophysiology.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81300044, 31670928, 81871356, 81770253, 81370362, 81871328, 81900047]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7162069, 7182149]; Beijing Municipal Administration of Hospitals' Youth Programme [QML20160301]; Open Foundation from Beijing Key Laboratory of Hypertension Research [2018GXY-KFKT-02]; National Key Research and Development Program of China [2016YFC0905600]; National Major Research Plan Training Program of China [91849111]; Chinese Academy of Medical Sciences Central Public-interest Scientific Institution Basal Research Fund Young Medical Talents Award Project [2018RC320013]
第一作者单位：[1]Medical Research Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China[2]Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Institute of Respiratory Medicine, Beijing 100020, China
通讯作者：
通讯机构：[2]Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Institute of Respiratory Medicine, Beijing 100020, China[4]Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
推荐引用方式(GB/T 7714)：
Miao Ran,Dong Xingbei,Gong Juanni,et al.Cell landscape atlas for patients with chronic thromboembolic pulmonary hypertension after pulmonary endarterectomy constructed using single-cell RNA sequencing[J].AGING-US.2021,13(12):16485-16499.doi:10.18632/aging.203168.
APA：
Miao Ran,Dong Xingbei,Gong Juanni,Li Yidan,Guo Xiaojuan...&Yang Yuanhua.(2021).Cell landscape atlas for patients with chronic thromboembolic pulmonary hypertension after pulmonary endarterectomy constructed using single-cell RNA sequencing.AGING-US,13,(12)
MLA：
Miao Ran,et al."Cell landscape atlas for patients with chronic thromboembolic pulmonary hypertension after pulmonary endarterectomy constructed using single-cell RNA sequencing".AGING-US 13..12(2021):16485-16499
医学